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Cell surface antigens coded for by the human chromosome 7

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Abstract

Human-mouse somatic cell hybrids, containing chromosome 7 from an SV40-transformed human cell line as the only human chromosome, were injected into the same inbred strain of mouse as the mouse parental cell, and the humoral immune response assayed. A cell-surface antigen(s) coded for by the chromosome 7 common to all human fibroblastic cell lines tested and also found on African green monkey kidney cell lines was demonstrated. No reactivity to SV40-induced TSTA was detected.

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Abbreviations

SV40:

simian virus 40

MEM:

minimal essential medium

FBS:

fetal bovine serum

FITC:

fluorescein isothiocyanate

C121:

53-87 (1) clone 21

Cl36:

53-87-3 clone 36

52-62:

52-62 (1) clone 5 subclone 9

MSV:

murine sarcoma virus

T:

tumor

TSTA:

tumor-specific transplantation antigen

RIA:

radioimmunoassay

PBS:

phosphate-buffered saline

PBS+ :

PBS with sodium azide and FBS

IgG:

immunoglobulin

cpm:

counts per minute

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  1. The Wistar Institute of Anatomy and Biology, 19104, Philadelphia, Pennsylvania

    David P. Aden & Barbara B. Knowles

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  1. David P. Aden
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  2. Barbara B. Knowles
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Aden, D.P., Knowles, B.B. Cell surface antigens coded for by the human chromosome 7. Immunogenetics 3, 209–221 (1976). https://doi.org/10.1007/BF01576954

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  • DOI: https://doi.org/10.1007/BF01576954

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