Skip to main content
Log in

Biodiversity of microorganisms that degrade bacterial and synthetic polyesters

  • Published:
Journal of Industrial Microbiology

Abstract

The biodiversity and occurrence in nature of bioplastic-degrading microorganisms are exemplified by the identification of 695 strains, isolated from different environments, such as soils, composts, natural waters, and sludge, that are able to degrade the bacterial polyester poly(3-hydroxybutyrate)in vitro. These microorganisms belong to at least 57 different taxa, including Gram-negative and Gram-positive bacteria, streptomycetes, and moulds. The literature on the biodiversity of poly(3-hydroxybutyrate)-degrading microorganisms is reviewed. The degrading abilities of 171 streptomycete strains were investigated on four different bacterial poly(3-hydroxyalkanoates), and the synthetic polyesters poly(ε-caprolactone) and BIONOLLE, and most of these strains degraded at least three different polymers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Anderson AJ and EA Dawes. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 54: 450–472.

    PubMed  Google Scholar 

  2. Bastioli C, A Cerutti, I Guanella, GC Romano and M Tosin. 1995. Physical state and biodegradation behavior of starch-polycaprolactone systems. J Environ Polym Degrad 3: 81–96.

    Google Scholar 

  3. Bernardet J-F, P Segers, M Vancanneyt, F Berthe, K Kersters and P Vandamme. 1996 Cutting a Gordian knot: emended classification and description of the genusFlavobacterium, emended description of the familyFlavobacteriaceae, and proposal ofFlavobacterium hydatis nom rev (basonym,Cytophaga aquatilis Strohl and Tait) 1978. Int J Syst Bacteriol 46: 128–148.

    Google Scholar 

  4. Brandl H, R Bachofen, J Mayer and E Wintermantel. 1995. Degradation and applications of polyhydroxyalkanoates. Can J Microbiol 41 (Suppl 1): 143–153.

    Google Scholar 

  5. Brandl H and P Püchner. 1992. Biodegradation of plastic bottles made from “Biopol’ in an aquatic ecosystem underin situ conditions. Biodegradation 2: 237–243

    Google Scholar 

  6. Brucato CL and SS Wong. 1991. Extracellular poly(3-hydroxybutyrate) depolymerase fromPenicillium funiculosum: general characteristics and active site studies. Arch Biochem Biophys 290: 497–502.

    PubMed  Google Scholar 

  7. Budwill K, PM Fedorak and WJ Page. 1992. Methanogenic degradation of poly(3-hydroxyalkanoates). Appl Environ Microbiol 58: 1398–1401.

    Google Scholar 

  8. Chowdbury AA. 1963. Poly-β-hydroxybuttersäure abbauende Bakterien und Exoenzym. Arch Mikrobiol 47: 167–200.

    PubMed  Google Scholar 

  9. Delafield FP, M Doudoroff, NJ Palleroni, J Lusty and R Contopoulos. 1965. Decomposition of poly-β-hydroxybutyrate by pseudomonads. J Bacteriol 90: 1455–1466.

    PubMed  Google Scholar 

  10. Doi Y. 1990. Microbial Polyesters. VCH Publishers, New York.

    Google Scholar 

  11. Gauthier G, M Gauthier and R Christen. 1995. Phylogenetic analysis of the generaAlteromonas, Shewanella, andMoritella using genes coding for small-subunit rRNA sequences and division of the genusAlteromonas into two genera,Alteromonas (emended) andPseudoalteromonas gen nov, and proposal of twelve new species combinations. Int J Syst Bacteriol 45: 755–761.

    PubMed  Google Scholar 

  12. Goldberg D. 1995. A review of the biodegradability and utility of poly(caprolactone). J Environ Polym Degrad 3: 61–68.

    Google Scholar 

  13. Haywood GW, AJ Anderson, DR Williams, EA Dawes and DF Ewing. 1991. Accumulation of a poly(hydroxyalkanoate) copolymer containing primarily 3-hydroxyvalerate from simple carbohydrate substrates byRhodococcus sp NCIMB 40126. Int J Biol Macromol 13: 83–88.

    PubMed  Google Scholar 

  14. Hrabak O. 1992. Industrial production of poly-β-hydroxybutyrate. FEMS Microbiol Rev 103: 251–256.

    Google Scholar 

  15. Jaeger K-E, A Steinbüchel and D Jendrossek. 1995. Substrate specificities of bacterial polyhydroxyalkanoate depolymerases and lipases: bacterial lipases hydrolyse poly(ω-hydroxyalkanoates). Appl Environ Microbiol 61: 3113–3118.

    PubMed  Google Scholar 

  16. Janssen PH and CG Harfoot. 1990.Ilyobacter delafieldii sp nov, a metabolically restricted anaerobic bacterium fermenting PHB. Arch Microbiol 154: 253–259.

    Google Scholar 

  17. Jendrossek D, A Frisse, A Behrends, M Andermann, HD Kratzin, T Stanislawski and HG Schlegel. 1995. Biochemical and molecular characterization of thePseudomonas lemoignei polyhydroxyalkanoate depolymerase system. J Bacteriol 177: 596–607.

    PubMed  Google Scholar 

  18. Jendrossek D, I Knoke, RB Habibian, A Steinbüchel and HG Schlegel. 1993. Degradation of poly(3-hydroxybutyrate), PHB, by bacteria and purification of a novel PHB depolymerase fromComamonas sp J Environ Polym Degrad 1: 53–64.

    Google Scholar 

  19. Matavulj M and HP Molitoris. 1992. Fungal degradation of polyhydroxyalkanoates and a semiquantitative assay for screening their degradation by terrestral fungi. FEMS Microbiol Rev 103: 323–332.

    Google Scholar 

  20. Matavulj M, ST Moss and HP Molitoris. 1993. Degradation of poly-β-hydroxyalkanoate-based plastics in natural environments. In: Proceedings of the International Symposium on Bacterial Polyhydroxyalkanoates. Göttingen, 1992 (Schlegel HG and A Steinbüchel, eds), pp 465–466, Goltze-Drück, Göttingen.

    Google Scholar 

  21. McLellan DW and PJ Halling. 1988. Acid-tolerant poly(3-hydroxybutyrate) hydrolases from moulds. FEMS Microbiol Lett 52: 215–218.

    Google Scholar 

  22. Mergaert J, C Anderson, A Wouters and J Swings. 1994 Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in compost. J Environ Polym Degrad 2: 177–183.

    Google Scholar 

  23. Mergaert J, C Anderson, A Wouters, J Swings and K Kersters 1992. Biodegradation of polyhydroxyalkanoates. FEMS Microbiol Rev 103: 317–322.

    Google Scholar 

  24. Mergaert J, G Glorieux, L Hauben, V Storms, M Mau and J Swings. 1996. Biodegradation of poly(3-hydroxyalkanoates) in anaerobic sludge and characterization of a poly(3-hydroxyalkanoates) degrading anaerobic bacterium. Syst Appl Microbiol (in press).

  25. Mergaert J, A Schirmer, L Hauben, M Mau, B Hoste, K Kersters, D Jendrossek and J Swings. 1996. Isolation and identification of poly(3-hydroxyvalerate) degrading strains ofPseudomonas lemoignei. Int J Syst Bacteriol 46: 769–773.

    PubMed  Google Scholar 

  26. Mergaert J, A Webb, C Anderson, A Wouters and J Swings. 1993. Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soils. Appl Environ Microbiol 59: 3233–3238.

    PubMed  Google Scholar 

  27. Mergaert J, A Wouters, C Anderson and J Swings. 1995.In situ biodegradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in natural waters. Can J Microbiol 41 (Suppl 1): 154–159.

    PubMed  Google Scholar 

  28. Mergaert J, A Wouters and J Swings. 1994. Estimation of the intrinsic biodiversity among poly(3-hydroxyalkanoates) degrading streptomycetes using gas chromatographic analysis of fatty acids. Syst Appl Microbiol 17: 601–612.

    Google Scholar 

  29. Mukai K, K Yamada and Y Doi. 1993. Enzymatic degradation of poly(hydroxybutyrate) by a marine microorganism. Polym Degrad Stab 41: 85–91.

    Google Scholar 

  30. Mukai K, K Yamada and Y Doi. 1994. Efficient hydrolysis of polyhydroxyalkanoates byPseudomonas stutzeri YM1414 isolated from lake water. Polym Degrad Stab 43: 319–327.

    Google Scholar 

  31. Nishida H and Y Tokiwa. 1993. Distribution of poly(β-hydroxybutyrate) and poly(ε-caprolactone) aerobic degrading microorganisms in different environments. J Environ Polym Degrad 1: 227–233.

    Google Scholar 

  32. Nishida H and Y Tokiwa. 1994. Degradation of poly(2-oxepanone) by phytopathogens. Chem Lett 1994: 1547–1550.

    Google Scholar 

  33. Ramsay BA, I Saracovan, JA Ramsay and RH Marchessault. 1994. A method for the isolation of microorganisms producing extracellular long-side-chain poly(β-hydroxyalkanoate) depolymerase. J Environ Polym Degrad 2: 1–7.

    Google Scholar 

  34. Schirmer A, D Jendrossek and HG Schlegel. 1993. Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase fromPseudomonas fluorescens GK13. Appl Environ Microbiol 59: 1220–1227.

    PubMed  Google Scholar 

  35. Schirmer A, C Matz and D Jendrossek. 1995 Substrate specificities of poly(hydroxyalkanoate) degrading bacteria and active site studies on the extracellular poly(3-hydroxyoctanoic acid) depolymerase ofPseudomonas fluorescens GK13. Can J Microbiol 41 (Suppl 1): 170–179.

    Google Scholar 

  36. Stanier RY, NJ Palleroni and M Doudoroff. 1966. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43: 159–271.

    PubMed  Google Scholar 

  37. Steinbüchel A. 1991. Polyhydroxyalkanoic acids. In: Biomaterials (Byrom D, ed), pp 123–213, Macmillan Press, London.

    Google Scholar 

  38. Steinbüchel A and G Schmack. 1995. Large-scale production of poly(3-hydroxyvaleric acid) by fermentation ofChromobacterium violaceum, processing, and characterization of the homopolyester. J Environ Polym Degrad 3: 243–258.

    Google Scholar 

  39. Steinbüchel A and HE Valentin. 1995. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol Lett 128: 219–228.

    Google Scholar 

  40. Tanio T, T Fukui, T Saito, K Tomita, T Kaito and S Masamune. 1982. An extracellular poly(3-hydroxybutyrate) depolymerase fromAlcaligenes faecalis. Eur J Biochem 124: 71–77.

    PubMed  Google Scholar 

  41. Timm A and A Steinbüchel. 1990. Formation of polyesters consisting of medium-chain length 3-hydroxyalkanoic acids from gluconate byPseudomonas aeruginosa and other fluorescent pseudomonads. Appl Environ Microbiol 56: 3360–3367.

    PubMed  Google Scholar 

  42. Yabuuchi E, Y Kosako, I Yano, H Hotta and Y Nishiushi. 1995. Transfer of twoBurkholderia and anAlcaligenes species toRalstonia gen nov: proposal ofRalstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb nov,Ralstonia solanacearum (Smith 1896) comb nov, andRalstonia eutropha (Davis 1969) comb nov. Microbiol Immunol 39: 897–904.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mergaert, J., Swings, J. Biodiversity of microorganisms that degrade bacterial and synthetic polyesters. Journal of Industrial Microbiology & Biotechnology 17, 463–469 (1996). https://doi.org/10.1007/BF01574777

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01574777

Keywords

Navigation