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Identification and quantitation of human T-cell antigen by antisera purified from antibodies crossreacting with hemopoietic progenitors and other blood cells

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Abstract

Anti-T cell globulin (ATCG) prepared from antihuman thymocyte serum by absorption with kidney, cells from patients with chronic lymphatic leukemias, and several lymphoblastoid cell lines was shown to react specifically with human thymus-derived lymphocytes. While high activity against thymocytes and a T-lymphoblastoid cell line could be demonstrated, ATCG remained negative against several chronic lymphatic leukemias and B-lymphoblastoid cell lines. The ATCG was used in the cytotoxic test, electronmicroscopy, and immunoautoradiography for identification of T cells in thymus, tonsils, spleen, blood, bone marrow, lymphatic leukemias, and lymphoblastoid cell lines. A comparison of these results with the ability to form spontaneous SRBC-rosettes revealed remarkable deviations between both markers in leukemias. Absorption with human brain failed to remove specific activity of ATCG. Labeling experiments by immunoautoradiography and investigations by complement fixation permitted quantitation of relative T-cell antigen concentration on different cell populations. As further evidence for specificity it could be shown that ATCG was no longer toxic for hemopoietic progenitors, whereas unabsorbed globulin reduced the number of colonyforming cells considerably.

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Abbreviations

ALL:

acute lymphatic leukemia

ATCG:

anti-human T cell globulin (absorbed)

ATG:

anti-human thymocyte globulin (not absorbed)

Bm-C:

bone marrow cells

CFA:

complete Freund's adjuvant

CLL:

chronic lymphatic leukemia

EBV:

Epstein-Barr virus

GPC':

guinea pig complement

HBSS:

Hanks' balanced salt solution

Ig:

immunoglobulin

PBS:

phosphate buffered saline

Per-Ly:

peripheral blood lymphocytes (normal)

Spl-Ly:

spleen lymphocytes

SRBC:

sheep red blood cells

VBS:

veronal buffered saline

Thy-Ly:

thymus lymphocytes

Ton-Ly:

tonsil lymphocytes

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Authors and Affiliations

  1. Institut für Hämatologie der Gesellschaft für Strahlen- und Umweltforschung mbH, Landwehrstraße 61, D-8000, München 2, Federal Republic of Germany

    Hans Rodt, Stefan Thierfelder, Eckhard Thiel, Dietrich Götze, Bruno Netzel, Dieter Huhn & Manfred Eulitz

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  1. Hans Rodt
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  2. Stefan Thierfelder
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  3. Eckhard Thiel
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  4. Dietrich Götze
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  5. Bruno Netzel
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  6. Dieter Huhn
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  7. Manfred Eulitz
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Rodt, H., Thierfelder, S., Thiel, E. et al. Identification and quantitation of human T-cell antigen by antisera purified from antibodies crossreacting with hemopoietic progenitors and other blood cells. Immunogenetics 2, 411–430 (1975). https://doi.org/10.1007/BF01572312

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