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Fumonisin-stimulatedN-acetyldihydrosphingosine,N-acetylphytosphingosine, and phytosphingosine products ofPichia (Hansenula) ciferri, NRRL Y-1031

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Abstract

Pichia (Hansenula) ciferri Y-1031 grown in the presence of 25–100 mg fumonisin B1/L for 4–5 days accumulated sphingolipids as evident in the centrifuged cells and extracellular particles (c/p fraction). The c/p fraction of fumonisin-treated (100 mg/L) cultures elicited a 15-fold increase ofN-acetyldihydrosphingosine and 31-fold increase of combinedN-acetylphytosphingosine and phytosphingosine over those from untreated cultures. During exponential growth of 1 day, fumonisin-treated cultures appeared to transfer sphingolipid bases into the medium (22 mg/L) rather than into the c/p (2 mg) fraction. Upon saponification, a residue from the c/p fraction contained 440 mg of additional, unknown polar lipids per liter that was not sphingolipid (14 mg/L).

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Authors and Affiliations

  1. Oil Chemical Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA

    Tsuneo Kaneshiro

  2. Mycotoxin Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA

    Ronald F. Vesonder

  3. Bioactive Constituents, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA

    Robert E. Peterson

Authors
  1. Tsuneo Kaneshiro
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  2. Ronald F. Vesonder
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  3. Robert E. Peterson
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Kaneshiro, T., Vesonder, R.F. & Peterson, R.E. Fumonisin-stimulatedN-acetyldihydrosphingosine,N-acetylphytosphingosine, and phytosphingosine products ofPichia (Hansenula) ciferri, NRRL Y-1031. Current Microbiology 24, 319–324 (1992). https://doi.org/10.1007/BF01571101

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