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The insecticidal crystal protein gene is expressed in vegetative cells ofBacillus thuringiensis var.tenebrionis

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Abstract

The time of appearance of the insecticidal crystal protein (ICP) transcript and the corresponding crystal antigen of the coleopteran-toxicBacillus thuringiensis var.tenebrionis was determined. The results indicate that inB. thuringiensis var.tenebrionis, unlike otherB. thuringiensis varieties, the ICP antigen and the ICP transcript are made at detectable levels in vegetative cells. The expression of the ICP gene increases dramatically and reaches a plateau in early stationary cells. In addition, results from immunoblotting analysis also suggest that the ICP antigen is made in vegetative cells as a 73-kilodalton peptide, which by proteolytic processing is converted to a 65-kilodalton peptide to some extent in stationary cells.

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  1. Agrigenetics Advanced Science Company, Madison, Wisconsin, USA

    Vaithilingam Sekar

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  1. Vaithilingam Sekar
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Sekar, V. The insecticidal crystal protein gene is expressed in vegetative cells ofBacillus thuringiensis var.tenebrionis . Current Microbiology 17, 347–349 (1988). https://doi.org/10.1007/BF01570876

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