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Influence of oxygen on thePseudomonas aeruginosa hydrogen cyanide synthase

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Abstract

Partially purified HCN Synthase (HCS) required exogenous electron acceptors for activity. Phenazine methosulfate (PMS) provided the greatest activity, whereas oxygen allowed only a limited response. TheP. aeruginosa secondary metabolite pyocyanin supported HCS-mediated cyanide production. HCN production by whole cells operated maximally at low oxygen levels, whereas moderate oxygen levels limited HCS activity. Respiration and cyanogenesis by whole cells were equally sensitive to azide; HCS was completely resistant.

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Authors and Affiliations

  1. Department of Biological Sciences, Duquesne University, 15282, Pittsburgh, Pennsylvania, USA

    Peter Castric

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  1. Peter Castric
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Castric, P. Influence of oxygen on thePseudomonas aeruginosa hydrogen cyanide synthase. Current Microbiology 29, 19–21 (1994). https://doi.org/10.1007/BF01570186

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  • DOI: https://doi.org/10.1007/BF01570186

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