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A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis inEscherichia coli

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Journal of Industrial Microbiology

Abstract

Different approaches to increasing carbon commitment to aromatic amino acid biosynthesis were compared in isogenic strains ofEscherichia coli. In a strain having a wild-type PEP: glucose phosphotransferase (PTS) system, inactivation of the genes encoding pyruvate kinase (pykA andpykF) resulted in a 3.4-fold increase in carbon flow to aromatic biosynthesis. In a strain already having increased carbon flow to aromatics by virtue of overexpression of thetktA gene (encoding transketolase), thepykA and/orpykF mutations had no effect. A PTS glucose+ mutant showed a 1.6-fold increase in carbon flow to aromatics compared to the PTS+ control strain. In the PTS− glucose+ host background, overexpression oftktA caused a further 3.7-fold increase in carbon flow, while inactivation ofpykA andpykF caused a 5.8-fold increase. When all of the variables tested (PTS− glucose+,pykA, pykF, and overexpressedtktA) were combined in a single strain, a 19.9-fold increase in carbon commitment to aromatic biosynthesis was achieved.

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Authors and Affiliations

  1. Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250, Cuernavaca, Morelos, México

    G Gosset

  2. Genencor International, 925 Page Mill Road, 94304-1013, Palo Alto, CA, USA

    J Yong-Xiao & A Berry

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  1. G Gosset
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  2. J Yong-Xiao
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  3. A Berry
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Gosset, G., Yong-Xiao, J. & Berry, A. A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis inEscherichia coli . Journal of Industrial Microbiology 17, 47–52 (1996). https://doi.org/10.1007/BF01570148

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  • DOI: https://doi.org/10.1007/BF01570148

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