Abstract
The effect of inoculum level on xylitol production byCandida guilliermondii was evaluated in a rice straw hemicellulose hydrolysate. High initial cell density did not show a positive effect in this bioconversion since increasing the initial cell density from 0.67 g L−1 to 2.41 g L−1 decreased both the rate of xylose utilization and xylitol accumulation. The maximum xylitol yield (0.71 g g−1) and volumetric productivity (0.56 g L−1 h−1) were reached with an inoculum level of 0.9 g L−1. These results show that under appropriate inoculum conditions rice straw hemicellulose hydrolysate can be converted into xylitol by the yeastC. guilliermondii with efficiency values as high as 77% of the theoretical maximum.
Similar content being viewed by others
References
Barbosa MFS, MB De Medeiros, IM De Mancilha, H Shneider and H Lee. 1988. Screening of yeasts for production of xylitol fromd-xylose and some factors which affect xylitol yield inCandida guilliermondii. J Ind Microbiol 3: 241–251.
Cao NJ, R Tang, CS Gong and LF Chen. 1994. The effect of cell density on the production of xylitol fromd-xylose by yeast. Appl Biochem Biotechnol 45/46: 515–519.
Chan EC, PP Ueng and LF Chen. 1989. Environmental effects ond-xylose fermentation bySchizosaccharomyces pombe. Appl Biochem Biotechnol 20/21: 221–232.
Heikkilä H, J Nurmi, L Rahkila and M Töyrylä. 1992. United States Patent 5 081 026.
Lu J, LB Tsai, CS Gong and GT Tsao. 1995. Effect of nitrogen sources on xylitol production fromd-xylose byCandida sp L-102. Biotechnol Lett 17: 167–170.
Melaja AL and L Hämäläinen. 1977. Process of making xylitol. United States Patent 4 008 285.
Meyrial V, JP Delgenes, R Moletta and JM Navarro. 1991. Xylitol production fromd-xylose byCandida guilliermondii: fermentation behaviour. Biotechnol Lett 13: 281–286.
Nolleau V, L Preziosi-Belloy and JM Navarro. 1995. The reduction of xylose to xylitol byCandida guilliermondii andCandida parapsilosis: incidence of oxygen and pH. Biotechnol Lett 17: 417–422.
Nolleau V, L Preziosi-Belloy, JP Delgenes and JM Navarro. 1993. Xylitol production from xylose by two yeast strains: sugar tolerance. Curr Microbiol 27: 191–197.
Roberto IC, S Sato, IM De Mancilha and MES Taqueda. 1995. Influence of media composition on xylitol fermentation byCandida guilliermondii using response surface methodology. Biotechnol Lett 17: 1223–1228.
Roberto IC, SS Silva, MGA Felipe, IM De Mancilha and S Sato. 1996. Bioconversion of rice straw hemicellulose hydrolysate for the production of xylitol: effect of pH and nitrogen source. Appl Biochem Biotechnol 57/58: 339–347.
Sreenath HK, TW Chapman and TW Jeffries. 1986. Ethanol production fromd-xylose in batch fermentations withCandida shehatae: process variables. Appl Microbiol Biotechnol 24: 294–299.
Vandeska E, S Amartey, S Kuzmanova and T Jeffries. 1995. Effects of environmental conditions on production of xylitol byCandida boidinii. World J Microbiol Biotechnol 14: 46–51.
Woods MA and NF Millis. 1985. Effect of slow feeding of xylose on ethanol yield byPachysolen tannophilus. Biotechnol Lett 7: 679–682.
Ylikahri R. 1979. Metabolic and nutritional aspects of xylitol. Adv Food Res 25: 159–180.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roberto, I.C., Sato, S. & de Mancilha, I.M. Effect of inoculum level on xylitol production from rice straw hemicellulose hydrolysate byCandida guilliermondii . Journal of Industrial Microbiology 16, 348–350 (1996). https://doi.org/10.1007/BF01570113
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01570113