Skip to main content
SpringerLink
Log in

Cu uptake in a cyanobacterium: Fate of selected photochemical reactions

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Cu uptake in the diazotrophic cyanobacteriumNostoc calcicola Bréb. was accompanied by inhibitions in the in vivo activities of photosystem (PS) II, PS I,14CO2-fixation, and decline in the ATP pool. Cyanobacterial cells, while saturated for Cu uptake within 1 h at 40 μM Cu, showed more than 50% inhibition of PS II and 95.4% of14CO2 fixation compared with only 15.5% decrease in the PS I activity. The total extractable ATP content also declined by 32.2% within 1 h. In a subsequent follow-up study lasting 72 h, PS II activity and14CO2 fixation showed complete inhibition, in contrast to 34.4% of PS I activity and 4.2% of ATP still remaining unaffected. The results have been discussed in the light of multiple effects of Cu during and subsequent to its uptake by the cyanobacterium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Allen MB, Arnon DI (1955) Studies on nitrogen fixing bluegreen algae. I. Growth and nitrogen fixation byAnabaena cylindrica Lemm. Plant Physiol 30:366–372

    Google Scholar 

  2. Asthana RK, Pandey PK, Singh SP (1990) Nickel regulation of photoautotrophy in a cyanobacterium. Water Air Soil Pollut 52:263–276

    Google Scholar 

  3. Bottomley PJ, Stewart WDP (1976) ATP pools and transients in the blue-green algaAnabaena cylindrica. Arch Microbiol 108:249–258

    PubMed  Google Scholar 

  4. Campbell PM, Smith GD (1986) Transport and accumulation of nickel ions in the cyanobacterium,Anabaena cylindrica. Arch Biochem Biophys 244:470–477

    PubMed  Google Scholar 

  5. Cedeno-Maldonado A, Swader JA, Heath RL (1972) The cupric ion as an inhibitor of photosynthetic electron transport in isolated chloroplasts. Plant Physiol 50:698–701

    Google Scholar 

  6. Herbert DP, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, Vol VB. London and New York: Academic Press, pp 209–344

    Google Scholar 

  7. Husaini Y, Singh AK, Rai LC (1991) Cadmium toxicity to photosynthesis and associated electron transport system ofNostoc linckia. Bull Environ Contam Toxicol 46:146–150

    PubMed  Google Scholar 

  8. Irmer U, Wachholz I, Schafer H, Lorch DW (1986) Influence of lead onChlamydomonas reinhardtii Danegard (volvocales, chlorophyta): accumulation, toxicity and ultrastructural changes. Environ Exp Bot 26:97–105

    Google Scholar 

  9. Larsson CM, Olsson T (1979) Firefly assay of adenine nucleotide from algae: comparison of extraction methods. Plant Cell Physiol 20:145–155

    Google Scholar 

  10. Les A, Walker RW (1984) Toxicity and binding of copper, zinc and cadmium by the blue-green alga,Chroococcus paris. Water Air Soil Pollut 23:129–139

    Google Scholar 

  11. Lowry OH, Rosebrough NJ, Farr AL Randall RJ (1951) Protein measurements with Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  Google Scholar 

  12. Murthy SDS, Sabat SC, Mohanty P (1989) Mercury-induced inhibition of photosystem II activity and changes in the emission of fluorescence from phycobilisomes in intact cells of the cyanobacterium,Spirulina platensis. Plant Cell Physiol 30:1153–1157

    Google Scholar 

  13. Murthy SDS, Bukhov NG, Mohanty P (1990) Mercury-induced alterations of chlorophylla fluorescence kinetics in cyanobacteria: multiple effects of mercury on electron transport. J Photochem Photobiol B Biol 6:373–380

    Google Scholar 

  14. Overnell J (1975) The effects of heavy metals on photosynthesis and loss of cell potassium in two species of marine algaeDunaliella tertiolecta andPhaeodactylum tricornutum. Marine Biol 29:99–103

    Google Scholar 

  15. Peter JN, Sherman LA (1978) Isolation and characterization of photosystem I and II membrane particles from the bluegreen alga,Synechococcus cedrorum. Biochim Biophys Acta 503:343–361

    PubMed  Google Scholar 

  16. Pettersson A, Bergmann B (1989) Effects of aluminium on ATP pools and utilization in the cyanobacteriumAnabaena cylindrica: a model for thein vivo toxicity. Physiol Plant 76:527–534

    Google Scholar 

  17. Pettersson A, Hällbom L, Bergman B (1988) Aluminium effects on uptake and metabolism of phosphorus by the cyanobacteriumAnabaena cylindrica. Plant Physiol 86:112–116

    Google Scholar 

  18. Robinson SJ, DeRoo CS, Yocum CF (1982) Photosynthetic electron transfer in preparations of the cyanobacteriumSpirulina platensis. Plant Physiol 70:154–161

    Google Scholar 

  19. Schecher WD, Driscoll CT (1985) Interactions of copper and lead withNostoc muscorum. Water Air Soil Pollut 24:85–101

    Google Scholar 

  20. Shehata FHA, Whitton BA (1982) Zinc tolerance in strains of blue-green algaAnacystic nidulans. Br Phycol J 17:5–12

    Google Scholar 

  21. Shioi Y, Tamai H, Sasa T (1978) Effect of copper on photosynthetic electron transport system in spinach chloroplast. Plant Cell Physiol 19:203–209

    Google Scholar 

  22. Singh CB, Singh SP (1987) Effect of mercury on photosynthesis inNostoc calcicola: role of ATP and interacting heavy metal ions. J Plant Physiol 129:49–58

    Google Scholar 

  23. Singh CB, Singh SP (1990) Hg2+-induced leakage of electrolytes and inhibition of NO −3 utilization inNostoc calcicola: role of interacting cations. Biol Metals 3:208–212

    Google Scholar 

  24. Singh DP (1985) Cu2+ transport in the unicellular cyanobacteriumAnacystic nidulans. J Gen Appl Microbiol 31:277–284

    Google Scholar 

  25. Singh DP, Singh SP (1987) Action of heavy metals on Hill activity and O2-evolution inAnacystis nidulans. Plant Physiol 83:12–14

    Google Scholar 

  26. Singh SP, Yodava V (1983) Cadmium induced inhibition of nitrate uptake inAnacystis nidulans: interaction with other divalent cations. J Gen Appl Microbiol 29:297–304

    Google Scholar 

  27. Singh SP, Yadava V (1984) Cadmium induced inhibition of ammonium and phosphate uptake inAnacystis nidulans: interaction with other divalent cations. J Gen Appl Microbiol 30:79–86

    Google Scholar 

  28. Singh SP, Yadava V (1985) Cadmium uptake inAnacystis nidulans: effect of modifying factors. J Gen Appl Microbiol 31:39–48

    Google Scholar 

  29. Singh DP, Khare P, Bisen PS (1989) Effect of Ni2+, Hg2+ and Cu2+ on growth, oxygen evolution and photosynthetic electron transport inCylindrospermum IU 942. J Plant Physiol 134:406–412

    Google Scholar 

  30. Stratton GW, Huber AL, Corke CT (1979) Effect of mercuric ion on growth, photosynthesis and nitrogenase activity ofAnabaena inaequalis. Appl Environ Microbiol 38:537–543

    PubMed  Google Scholar 

  31. Verma SK, Singh SP (1990) Factors regulating copper uptake in a cyanobacterium. Curr Microbiol 21:33–37

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Algal Research Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, 221005, Varanasi, India

    P. K. Pandey, C. B. Singh &  S. P. Singh

Authors
  1. P. K. Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. B. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. P. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pandey, P.K., Singh, C.B. & Singh, S.P. Cu uptake in a cyanobacterium: Fate of selected photochemical reactions. Current Microbiology 24, 35–39 (1992). https://doi.org/10.1007/BF01570097

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01570097

Keywords

Search

Navigation