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The effect of a propionic acid bacterial inoculant applied at ensiling on the aerobic stability of wheat and sorghum silages

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Journal of Industrial Microbiology

Abstract

The effect of a new strain ofPropionibacterium shermanii (PAB), applied at ensiling, on the aerobic stability of wheat and sorghum silages was studied in several experiments under laboratory conditions. In the one experiment with wheat and in those with sorghum a lactic acid bacteria (LAB) inoculant (Lactobacillus plantarum andPediococcus cerevisiae) was also included. After treatment, the chopped forages were ensiled in 1.5-L anaerobic jars which were sampled in triplicate on predetermined dates to follow fermentation dynamics. At the end of the experiments, the silages were subjected to an aerobic stability test. The PAB inoculant improved the aerobic stability only in one experiment with wheat, in which the decrease in pH was very slow; the final pH remained relatively high (4.5). The PAB-treated silages contained 19.5±2.0 g of propionic acid per kg of dry matter. In the experiments with sorghum, the control and PAB-inoculated silages were stable, whereas LAB-inoculated silages deteriorated. The results suggest that PAB can survive in and improve the aerobic stability of only slow-fermenting silages which are prone to aerobic deterioration.

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References

  1. Ashbell G, ZG Weinberg, A Azrieli, Y Hen and B Horev. 1991. A simple system to study aerobic deterioration of silages. Can Agric Engn 33: 391–395.

    Google Scholar 

  2. Barker SB and WH Summerson. 1941. The colorimetric determination of lactic acid in biological material. J Biol Chem 138: 535–554.

    Google Scholar 

  3. Dubois M, KA Giles, JK Hamilton, PA Rebes and F Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356.

    Google Scholar 

  4. Henderson AR, DR Seale, DH Anderson and SJE Heron. 1990. The effect of formic acid and bacterial inoculants on the fermentation and nutritive value of perennial ryegrass silages. In: Proceedings of the Eurobac Conference (Lindgren S and KL Petterson, eds), pp 93–98. Swedish University of Agricultural Sciences, Uppsala, Sweden, August 1986.

    Google Scholar 

  5. Kennedy SJ. 1990. Evaluation of three bacterial inoculants and formic acid as additives for first harvest grass. Grass and Forage Sci 45: 153–165.

    Google Scholar 

  6. Lindgren S, R Lingvall, A Kaspersson, A Kartzow and E Rydberg. 1983. Effects of inoculants, grain and formic acid on silage fermentation. Swedish J Agric Res 13: 91–100.

    Google Scholar 

  7. Moon NJ. 1983. Inhibition of the growth of acid tolerant yeasts by acetate, lactate and propionate, and their synergistic mixture. J Appl Bacteriol 55: 453–460.

    Google Scholar 

  8. Pahlow G and H Honig. 1994. The role of microbial additives in the aerobic stability of silage. In: Workshop Proceedings of the 15th General Meeting of the European Grassland Federation Wageningen, pp 149–151, The Netherlands, June 1994.

  9. Rust SR, HS Kim and GL Enders. 1989. Effects of a microbial inoculant on fermentation characteristics and nutritional value of maize silage. J Production Agric 2: 235–241.

    Google Scholar 

  10. Spoelstra SF. 1991. Chemical and biological additives in forage conservation. In: Proceedings of a Conference on Forage Conservation towards 2000 (Pahlow G and H Honig, eds), pp 48–70, Braunschweig, Germany, January 1991.

    Google Scholar 

  11. Theune HH. 1978. Gaschromatographische Bestimmung von kurzkettigen Fettsauren einschliesslich Athanol und Milchsaure in Garfutter. Ein Methodenvergleich. Landwirtschaftliche Forschung, Sonderheft 35, 90 VDLUFA Kongress, Augsburg, Germany, September 12–18.

  12. Weinberg ZG, G Ashbell and A Azrieli. 1988. The effect of applying lactic acid bacteria at ensilage on the chemical and microbiological composition of vetch, wheat and alfalfa silages. J Appl Bacteriol 64: 1–7.

    Google Scholar 

  13. Weinberg ZG, G Ashbell, A Azrieli and I Brukental. 1993a. Ensiling peas, ryegrass and wheat with additives of lactic acid bacteria (LAB) and cell wall degrading enzymes. Grass and Forage Sci 48: 70–78.

    Google Scholar 

  14. Weinberg ZG, G Ashbell, Y Hen and A Azrieli. 1993b. The effect of applying lactic acid bacteria at ensiling on the aerobic stability of silages. J Appl Bacteriol 75: 512–518.

    Google Scholar 

  15. Weinberg ZG, G Ashbell, KK Bolsen, G Pahlow, Y Hen and A Azrieli. 1995. The effect of a propionic acid bacterial inoculant applied at ensiling, with or without lactic acid bacteria, on the aerobic stability of pearl millet and maize silages. J Appl Bacteriol 78: 430–436.

    Google Scholar 

  16. Woolford MK. 1990. The detrimental effects of air on silage. J Appl Bacteriol 68: 101–116.

    PubMed  Google Scholar 

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Weinberg, Z.G., Ashbell, G., Hen, Y. et al. The effect of a propionic acid bacterial inoculant applied at ensiling on the aerobic stability of wheat and sorghum silages. Journal of Industrial Microbiology 15, 493–497 (1995). https://doi.org/10.1007/BF01570020

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  • DOI: https://doi.org/10.1007/BF01570020

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