Summary
Rhizopus arrhizus biomass attached itself to fermentor walls, baffles and impellers when grown in casein/ glucose media. In shake flasks, dispersed filamentous growth was produced in media containing certain concentrations of glucose and soya flour. Other media tested produced pelleted or clumpy growth. Medium initial pH did not affect morphology type. Dispersed growth could not be obtained by addition of detergents, oils and polymers to a clear glucose/soya peptone medium. Addition of maize solids to this medium resulted in dispersed growth which occurred even in the presence of calcium, which in most media caused pellet formation. Mycelia appeared to bind to the maize particles and use these as growth centres thereby preventing pellet or clump formation. Mycelial pellets appeared to originate either from a single spore or by interaction of branched hyphae from different spores. Medium composition and macro-morphology type correlate with differences in hyphal structures.
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References
Beuchat, L.R. 1984. Fermented soybean foods. Food Technol. 38: 64–70.
Byrne, G.S. and O.P. Ward. 1987. Effect of polymens on pelleting ofRhizopus arrhizus. Trans. Br. Mycol. Soc. 89: 367–371
Byrne, G.S. and O.P. Ward. 1988. Effect of nutrition on pellet formation byRhizopus arrhizus. Biotechnol. Bioeng., in press.
Calam, C.T. 1969. The evaluation of mycelial growth. In: Methods in Microbiology, Vol. 1 (Norris, J.R. and D.E. Ribbons, eds.), pp. 567–591, Academic Press, London.
Calam, C.T. 1976. Starting investigational and industrial cultures. Process Biochem. 11: 7–12.
Collins, C.H. and P.M. Lyne. 1979. Microbiological Methods, 4th Edn., Butterworth, London.
De Rowe, L. and G.M. Gadd. 1987. Copper adsorption byRhizopus arrhizus, Cladosporium resinae andPenicillium italicum. Appl. Microbiol. Biotechnol. 26: 84–90.
Dion, W.M., A. Carilli, G. Sermonti and E.B. Chain. 1954. The effect of mechanical agitation on the morphology ofPenicillium chrysogenum Thom in stirred fermenters. Rend. 1st Super Sanita 17: 187–205.
Duckworth, R.B. and G.C. Harris. 1949. The morphology ofPenicillium chrysogenum in submerged fermentations. Trans. Br. Mycol. Soc. 32: 224–235.
Elmayergi, H., J.M. Scharer and M. Moo-Young. 1973. Effects of polymer additives on fermentation parameters in a culture ofA. niger. Biotechnol. Bioeng. 15: 845–859.
Engelhardt, G. and P.R. Wallnofer. 1978., Microbial transformation of the triazinone herbicide metamitron to desaminometamitron. Chemosphere 7: 463.
Galbraith, J.C. and J.E. Smith. 1969. Filamentous growth ofAspergillus niger in submerged culture. Trans. Br. Mycol. Soc. 52: 237–246.
Godfrey, T. and J. Reichelt. 1981. Industrial Enzymology, MacMillan, London.
Huang, J.Y. and H.R. Bungay. 1963. Microprobe measurements of oxygen concentrations in mycelial pellets. Biotechnol. Bioeng. 15: 1193–1197.
Konig, B., K. Schügerl and C. Seewald. 1982. Strategies for penicillin fermentation in tower-loop fermenters. Biotechnol. Bioeng. 24: 259–280.
Laanio, T.L., P.C. Kearney and D.D. Kaufman. 1973. Microbial metabolism of dinitramine. Pestic. Biochem. Physiol. 3: 271–277.
Metz, B. and N.W.F. Kossen. 1977. Biotechnology Review. The growth of molds in the form of pellets—a literature review. Biotechnol. Bioeng. 19: 781–790.
Phillips, D.H. 1966. Oxygen transfer into mycelial pellets. Biotechnol. Bioeng. 8: 456–460.
Pirt, S.J. and D.S. Callow. 1959. Continuous flow culture of the filamentous mouldPenicillium chrysogenum and the control of its morphology. Nature 184: 307–310.
Rhodes, R.A., A.A. Lagoda, T.J. Misenheimer, M.L. Smith, R.F. Anderson and R.W. Jackson. 1962. Production of fumaric acid in 20-litre fermenters Appl. Microbiol. 10: 9–15.
Rhodes, R.A., A.J. Moyer, M.L. Smith and S.E. Kelley. 1959. Production of fumaric acid byRhizopus arrhizus. Appl. Microbiol. 7: 74–80.
Solomons, G.L. 1985. Production of biomass by filamentous fungi. In: Comprehensive Biotechnology, Vol. 1, (Moo-Young, M., ed.), pp. 483–505. Pergamon Press, Oxford.
Vezina, C., S. Rakhit and G. Medawar. 1977. In: Handbook of Microbiology, 2nd Edn., Vol. VII—Microbial Transformation (Laksin A.I. and H.A. Lechevalier, eds.), pp. 65–466, CRC, Boca Raton, FL.
Vining, L.C. 1977. In: Handbook of Microbiology, 2nd Edn., Vol. V—Microbial Products (Laskin, A.E. and H.A. Lechevalier, eds.), pp. 609–618, CRC, Boca Raton, FL.
Wallnofer, P.R., S. Safe and O. Hutzinger. 1972. Hydroxylation of the herbicide Karsil (N-(3,4-dichlorophenyl)-2-methylpentameamide) byRhizopus japonicus. Chemosphere 1: 153–158.
Wallnofer, P.R., F. Sohlemann and L. Oehlmann. 1976. Metabolism of fenthion byrhizopus japonicus. Pflanzenschutz Nachr. Bayer 29: 236–253.
Ward, O.P. 1988. Fermentation Biotechnology: Principles, Processes and Products, Open University Press, Milton Keynes.
Weete, J.D., J.L., Laseter and G.C. Lawler. 1973. Total lipid and sterol components ofRhizopus arrhizus: identification and metabolism. Arch. Biochem. Biophys. 155: 411–419.
Weete, J.D., J.D. Weber and J.L. Laseter. 1970. Lipids ofRhizopus arrhizus. J. Bacteriol. 103: 536–540.
Whitaker, A. and P.A. Long. 1973. Fungal pelleting. Process Biochem. 8: 27–31.
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Byrne, G.S., Ward, O.P. Growth ofRhizopus arrhizus in fermentation media. Journal of Industrial Microbiology 4, 155–161 (1989). https://doi.org/10.1007/BF01569800
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DOI: https://doi.org/10.1007/BF01569800