Summary
Although the addition of shikimic acid to the medium had no effect on the level of production of rapamycin byStreptomyces hygroscopicus,14C-shikimic acid was incorporated into rapamycin to a very high degree.13C-Shikimic acid was successfully prepared from 1-[13C]-glucose using a mutant ofKlebsiella pneumoniae, and used to label rapamycin. It was found that13C-shikimic acid was incorporated into the cyclohexane moiety of rapamycin, thereby establishing the shikimic acid pathway origin of the seven-carbon starter unit.
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Paiva, N.L., Roberts, M.F. & Demain, A.L. The cyclohexane moiety of rapamycin is derived from shikimic acid inStreptomyces hygroscopicus . Journal of Industrial Microbiology 12, 423–428 (1993). https://doi.org/10.1007/BF01569676
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DOI: https://doi.org/10.1007/BF01569676