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Isolation and characterization of a recombination defective-dependent bacteriophage ofRhodobacter sphaeroides

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Abstract

A new virulent bacteriophage, designated RZ1, was isolated from a local pond on the facultative phototrophic bacteriumRhodobacter sphaeroides ZZ101. Electron microscopic studies revealed that, in general morphology, phage RZ1 resembles the λ bacteriophage ofEscherichia coli. The host range of phage RZ1 is limited to some strains ofR. sphaeroides. The phage genome consists of double-stranded DNA of about 44 kb lacking cohesive ends and seems to present terminal redundancy and cyclic permutation. RZ1 phage may carry out a lytic cycle only in recombination-defective mutants ofR. sphaeroides. Nevertheless, a derivative of the RZ1 phage, termed RW1, able to grow in recombination-proficient strains ofR. sphaeroides, has also been obtained. In vitro restriction analysis of both RZ1 and RW1 phages shows the presence of a rearrangement in their DNA. Generalized transduction of Strr and Rifr chromosomal markers has not been detected with either RZ1 or RW1 phages.

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Authors and Affiliations

  1. Department of Genetics and Microbiology, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, 08193, Barcelona, Spain

    Eloi Garí, Manuel Ollé, Isidre Gibert, Montserrat Llagostera &  Jordi Barbé

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  1. Eloi Garí
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  2. Manuel Ollé
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  3. Isidre Gibert
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  4. Montserrat Llagostera
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  5. Jordi Barbé
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Garí, E., Ollé, M., Gibert, I. et al. Isolation and characterization of a recombination defective-dependent bacteriophage ofRhodobacter sphaeroides . Current Microbiology 24, 151–157 (1992). https://doi.org/10.1007/BF01568980

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