Skip to main content
Log in

A phylogenetic analysis of anaerobic eubacteria capable of synthesizing acetate from carbon dioxide

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Acetobacterium woodii, Acetogenium kivui, Clostridium aceticum, C. acidiurici, C. cylindrosporum, C. formicoaceticum, C. thermoaceticum, Eubacterium limosum, andPeptococcus glycinophilus were characterized by oligonucleotide cataloging of their 16S ribosomal RNA to determine whether the ability to synthesize acetate from CO2 is a phylogenetic trait. The ability to synthesize acetate from CO2 apparently is not a valid phylogenetic marker. TheEubacterium andPeptococcus species examined here are less related to other species in their genera than they are to different species ofClostridium. TheEubacterium species examined here show little relatedness to the genusPropionibacterium. The acetogenic eubacteria belong to the phylogenetic group defined basically by the Gram-positive sporeforming anaerobes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literature Cited

  1. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R., Wolfe, R. S. 1979. Methanogens: Reevaluation of a unique biological group. Microbiological Reviews43:260–296.

    PubMed  Google Scholar 

  2. Balch, W. E., Schoberth, S., Tanner, R. S., Wolfe, R. S. 1977.Acetobacterium, a new genus of hydrogen-oxidizing, carbon dioxide-reducing, anaerobic bacteria. International Journal of Systematic Bacteriology27:355–361.

    Google Scholar 

  3. Braun, M., Mayer, F., Gottschalk, G. 1981.Clostridium aceticum (Wieringa), a microorganism producing acetic acid from molecular hydrogen and carbon dioxide. Archives of Microbiology128:288–293.

    PubMed  Google Scholar 

  4. Fox, G. E., Pechman, K. R., Woese, C. R. 1977. Comparative cataloging of 16S ribosomal ribonucleic acid: Molecular approach to procaryotic systematics. International Journal of Systematic Bacteriology27:44–57.

    Google Scholar 

  5. Fox, G. E., Stackebrandt, E., Hespell, R. B., Gibson, J., Maniloff, J., Dyer, T. A., Wolfe, R. S., Balch, W. E., Tanner, R. S., Magrum, L. J., Zablen, L. B., Blakemore, R., Gupta, R., Bonen, L., Lewis, B. J., Stahl, D. A., Luehrsen, K. R., Chen, K. N., Woese, C. R. 1980. The phylogeny of prokaryotes. Science209:457–463.

    PubMed  Google Scholar 

  6. Johnson, J. L., Francis, B. S. 1975. Taxonomy of the clostridia: Ribosomal ribonucleic acid homologies among the species. Journal of General Microbiology88:229–244.

    PubMed  Google Scholar 

  7. Leigh, J. A., Mayer, F., Wolfe, R. S. 1981.Acetogenium kivui, a new thermophilic hydrogen-oxidizing, acetogenic bacterium. Archives of Microbiology129:275–280.

    Google Scholar 

  8. Ljungdahl, L. G., Andreesen, J. R. 1976. Reduction of CO2 to acetate in homoacetate fermenting clostridia and the involvement of tungsten in formate dehydrogenase, pp. 163–172. In: Schlegel, H. G., Gottschalk, G., Pfennig, N. (eds.), Microbial production and utilization of gases. Göttingen: E. Goltze K. G.

    Google Scholar 

  9. Ljungdahl, L. G., Wood, H. G. 1969. Total synthesis of acetate from CO2 by heterotrophic bacteria. Annual Review of Microbiology23:515–538.

    PubMed  Google Scholar 

  10. Moore, W. E. C., Holdeman, L. V. 1974.Propionibacteriaceae, pp. 633–657. In: Buchanian, R. E., Gibbons, N. E. (eds.), Bergey's manual of determinative bacteiology, 8th ed. Baltimore: Williams & Wilkins.

    Google Scholar 

  11. Pechman, K. R. 1976. Investigation of the phylogenetic relationship ofSporosarcina ureae to members of theBacillaceae using primary structural characterization of 16S ribosomal ribonucleic acids. Ph.D. thesis. University of Illinois, Urbana, Illinois.

    Google Scholar 

  12. Sharak Genthner, B. R., Davis, C. L., Bryant, M. P. 1981. Features of rumen and sewage sludge strains ofEubacterium limosum, a methanol- and H2−CO2-utilizing species. Applied and Environmental Microbiology42:12–19.

    PubMed  Google Scholar 

  13. Stackebrandt, E., Woese, C. R. 1981. Towards a phylogeny of the actinomycetes and related organisms. Current Microbiology5:197–202.

    Google Scholar 

  14. Tanner, R. S., Stackebrandt, E., Fox, G. E., Woese, C. R. 1981. A phylogenetic analysis ofAcetobacterium woodii, Clostridium barkeri, Clostridium butyricum, Clostridium lituseburense, Eubacterium limosum, andEubacterium tenue. Current Microbiology5:35–38.

    Google Scholar 

  15. Tanner, R. S., Wolfe, R. S., Ljungdahl, L. G. 1978. Tetrahydrofolate enzyme levels inAcetobacterium woodii and their implication in the synthesis of acetate from CO2. Journal of Bacteriology134:668–670.

    PubMed  Google Scholar 

  16. Uchida, T., Bonen, L., Schaup, H. W., Lewis, B. J., Zablen, L., Woese, C. R. 1974. The use of ribonuclease U2 in RNA sequence determination. Some corrections in the catalog of oligomers produced by ribonuclease T1 digestion ofEscherichia coli 16S ribosomal RNA. Journal of Molecular Evolution3:63–77.

    PubMed  Google Scholar 

  17. Waber, L. J., Wood, H. G. 1979. Mechanism of acetate synthesis from CO2 byClostridium acidiurici. Journal of Bacteriology140:468–478.

    PubMed  Google Scholar 

  18. Woese, C. R., Sogin, M., Stahl, D., Lewis, B. J., Bonen, L. 1976. A comparison of the 16S ribosomal RNAs from mesophilic and thermophillic bacilli: Some modifications in the Sanger method for RNA sequencing. Journal of Molecular Evolution7:197–213.

    PubMed  Google Scholar 

  19. Wolin, E. A., Wolin, M. J., Wolfe, R. S. 1963. Formation of methane by bacterial extracts. Journal of Biological Chemistry238:2882–2886.

    PubMed  Google Scholar 

  20. Zablen, L. 1976. Procaryotic phylogeny by ribonucleic acid sequence homology. Ph.D. thesis. University of Illinois, Urbana, Illinois.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanner, R.S., Stackebrandt, E., Fox, G.E. et al. A phylogenetic analysis of anaerobic eubacteria capable of synthesizing acetate from carbon dioxide. Current Microbiology 7, 127–132 (1982). https://doi.org/10.1007/BF01568961

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01568961

Keywords

Navigation