Skip to main content
SpringerLink
Log in

Plasmid profiles ofMycobacterium fortuitum complex isolates

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Six plasmids differing in molecular weights were found in isolates ofMycobacterium fortuitum, M. chelonae, and other nonclassified, nonchromogenic, rapidly growing mycobacteria. One of the plasmids of molecular weight of 32.0 Kb was present in all strains analyzed; a large plasmid of 112.0-Kb molecular weight was present only in strains identified asM. fortuitum var.peregrinum. Although the strains had distinct plasmid profiles, none of the 15 cultural and biochemical properties and susceptibility to 18 drugs that were tested could be attributed to the occurrence of these plasmids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Crawford, J. T., Bates, J. H. 1979. Isolation of plasmids from mycobacteria. Infection and Immunity24:979–981.

    PubMed  Google Scholar 

  2. Crawford, J. T., Cave, M. D., Bates, J. H. 1981. Characterization of plasmids from strains ofMycobacterium aviumintracellulare. Review of Infectious Diseases3:949–952.

    Google Scholar 

  3. Crawford, J. T., Cave, M. D., Bates, J. H. 1981. Evidence for plasmid-mediated restriction-modification inMycobacterium avium-intracellulare. Journal of General Microbiology127:333–338.

    PubMed  Google Scholar 

  4. David, H. L., Traoré, I., Feuillet, A. 1981. Differential identification ofMycobacterium fortuitum andMycobacterium chelonei. Journal of Clinical Microbiology13:6–9.

    PubMed  Google Scholar 

  5. Hanse, J. B., Olsen, R. H. 1978. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5. Journal of Bacteriology135: 227–238.

    PubMed  Google Scholar 

  6. Kleinschmidt, A. K. 1968. Monolayer technics in electron microscopy of nucleic acid molecules, pp. 361–377. In: Grossman, L., Moldave, L. (eds.) Methods in enzymology, vol. 12, New York: Academic Press.

    Google Scholar 

  7. Kubica, G. P., Baess, I., Gordon, R. E., Jenkins, P. A. Kwapinsky, J. B. G., McDurmont, C., Pattyn, S. R., Saito, H., Silcox, V., Stanford, J. L., Takeya, K., Tsukamura, M. 1972. A cooperative numerical analysis of rapidly growing mycobacteria. Journal of General Microbiology73:55–70.

    PubMed  Google Scholar 

  8. Lévy-Frébault, V., Daffé, M., Goh, K. S., Lanéelle, M.-A., Asselineau, C., David, H. L. 1983. Identification ofMycobacterium fortuitum andMycobacterium chelonei. Journal of Clinical Microbiology17:744–752.

    PubMed  Google Scholar 

  9. Meissner, P. S., Falkinham III, J. O. 1984. Plasmid-encoded mercuric reductase inMycobacterium scrofulaceum. Journal of Bacteriology157:669–672.

    PubMed  Google Scholar 

  10. Meyers, J. A., Sanchez, D., Elwell, L. P., Falkow, S. 1976. Simple agarose gel electrophoresis method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology127:1529–1537.

    PubMed  Google Scholar 

  11. Mizuguchi, Y., Fukunaga, M., Taniguchi, H. 1981. Plasmid deoxyribonucleic acid and translucent-to-opaque variation inMycobacterium intracellulare 103. Journal of Bacteriology146:656–659.

    PubMed  Google Scholar 

  12. Rastogi, N., David, H. L. 1981. Ultrastructural and chemical studies on wall-deficient forms, spheroplasts, and membrane vesicles fromMycobacterium aurum. Journal of General Microbiology124:71–79.

    PubMed  Google Scholar 

  13. Rastogi, N., David, H. L., Rafidinarivo, E. 1983. Spheroplast fusion as a mode of genetic recombination in mycobacteria. Journal of General Microbiology129:1227–1237.

    PubMed  Google Scholar 

  14. Rastogi, N., Lévy-Frébault, V., David, H. L. 1983. Spheroplast formation from nine rapidly growing mycobacteria. Current Microbiology9:201–204.

    Google Scholar 

  15. Redmond, W. B., Ward, D. M. 1966. Media and methods for phage typing mycobacteria. Bulletin of World Health Organization65:563–568.

    Google Scholar 

  16. Saito, H., Gordon, R. E., Juhlin, I., Kappler, W., Kwapinsky, J. B. G., McDurmont, C., Pattyn, S. R., Runyon, E. H., Stanford, J. L., Tarnok, I., Tasaka, H., Tsukamura, M. Weiszfeiler, J. 1977. Cooperative numerical analysis of rapidly growing mycobacteria. International Journal of Systematic Bacteriology27:75–85.

    Google Scholar 

  17. Silcox, V., Good, R. C., Floyd, M. M. 1981. Identification of clinically significantMycobacterium fortuitum complex isolates. Journal of Clinical Microbiology 14:686–691.

    PubMed  Google Scholar 

  18. Swenson, J. M., Thornsberry, C., Silcox, V. A. 1982. Rapidly growing mycobacteria: testing of susceptibility to 34 antimicrobial agents by broth microdilution. Antimcrobial Agents and Chemotherapy22:186–192.

    Google Scholar 

  19. Wallace, R. J., Dalovisio, J. R., Pankey, G. A. 1979. Disk diffusion susceptibility testing ofM. fortuitum andM. chelonei to antibacterial agents. Antimicrobial Agents and Chemotherapy16:611–614.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Service de la Tuberculose et des Mycobactéries, Institut Pasteur, 25 rue du Dr. Roux, Paris 15, France

    Abdelhakim Labidi, Khye Seng Goh & Hugo L. David

  2. Unit of Electron Microscopy, Department of Virology, Pasteur Institute, Paris, France

    Charles Dauguet

Authors
  1. Abdelhakim Labidi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Charles Dauguet
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Khye Seng Goh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hugo L. David
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Labidi, A., Dauguet, C., Goh, K.S. et al. Plasmid profiles ofMycobacterium fortuitum complex isolates. Current Microbiology 11, 235–240 (1984). https://doi.org/10.1007/BF01567167

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01567167

Keywords

Search

Navigation