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Growth of acetotrophic, methane-producing bacteria in a pH auxostat

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Abstract

Three acetotrophicMethanosarcina species, which included marine, nonmarine, and thermophilic strains, were grown on acetate in a 10-liter pH auxostat. Specific growth rates and molar growth yields were constant throughout growth. Cell yields were up to 18-fold greater than previously reported. These properties of the pH auxostat indicate that it is a preferred culture method for the biochemical study of methanogenesis from acetate.

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Authors and Affiliations

  1. Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA

    Kevin R. Sowers, Michael J. Nelson & James G. Ferry

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  1. Kevin R. Sowers
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  2. Michael J. Nelson
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  3. James G. Ferry
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Sowers, K.R., Nelson, M.J. & Ferry, J.G. Growth of acetotrophic, methane-producing bacteria in a pH auxostat. Current Microbiology 11, 227–229 (1984). https://doi.org/10.1007/BF01567165

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