Abstract
The cyanobacteriumAnabaena siamensis Antarikanonda, isolated from rice paddies of Bangkok, Thailand, liberates substantial quantities of free amino acids into the external medium irrespective of whether it is growing on N2, NH4 +, NO3 − or under nitrogen-starved conditions. Addition of such combined nitrogen causes changes in both intracellular and extracellular free amino acid pool patterns. No overall relationship exists between the amino acid efflux and the intracellular pools. The most abundant free amino acids found in the external media of N2, NO3 −, NH4 +-grown and N-starved cultures were phenylalanine, threonine, glutamate, and glycine, respectively. These investigations suggest that amino acid liberation by the cyanobacterium is a selective diffusional process that is sensitive to environmental changes.
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Literature Cited
Angadi, S. B., Bharati, S. G. 1983. Nitrogen fixation and production of extracellular nitrogen byHapalosiphon confervaceus Borzi andNostoc microscopicum Carm. ex Born. et Flah. Beiträge zur Biologie der Pflanzen58:205–209.
Antarikanonda, P. 1980. Eco-physiological, morphological and biochemical studies with the rapidly-growing, N2-fixing blue-green alga (cyanobacterium),Anabaena sp. TA 1 isolated from paddy soils of Bangkok, Thailand. PhD thesis, University of Göttingen.
Antarikanonda, P. 1982. Effect of salinity on growth, nitrogen fixation and sodium uptake of rapidly growing N2-fixing blue-green algaAnabaena sp. TA 1. Microbios34:177–184.
Antarikanonda, P. 1982. Influence of pretreating the seeds with extract ofAnabaena sp. TA 1 on germination and growth of some rice varieties from Thailand. International Rice Commission Newsletter31(2):37–39.
Antarikanonda, P. 1984. A new species of the genusAnabaena: Anabaena siamensis sp. nov. (Cyanophyceae) from Thailand. Nova Hedwigia (in press).
Antarikanonda, P., Lorenzen, H. 1982. N2-fixing blue-green algae (cyanobacteria) of high efficiency from paddy soils of Bangkok, Thailand: characterization of species and N2-fixing capacity in the laboratory. Archiv für Hydrobiologie/Supplimentband63, Algological Studies30:53–70.
Antarikanonda, P., Lorenzen, H. 1983. Highly efficient N2-fixing blue-green algae Thailands: a possible remedy to nitrogen scarcity. Bangkok MIRCEN Newsletter3(1):1–4.
Brown, A. W., Stanley, S. O. 1972. Environment-mediated changes in the cellular content of the ‘pool’ constituents and their associated changes in cell physiology. Journal of Applied Chemistry and Biotechnology22:363–389.
De, P. K. 1939. The role of blue-green algae in nitrogen fixation in rice fields. Proceedings of the Royal Society of London B127:121–139.
Dharmawardene, M. W. N., Stewart, W. D. P., Stanley, S. O. 1972. Nitrogenase activity, amino acid pool patterns and amination in blue-green algae. Planta108:133–145.
Fogg, G. E. 1952. The production of extracellular nitrogenous substrances by a blue-green alga. Proceedings of the Royal Society of London B139:372–397.
Fogg, G. E. 1966. The extracellular products of algae. Oceanography and Marine Biology4:195–212.
Fogg, G. E., Stewart, W. D. P., Fay, P., Walsby, A. E. 1973. The blue-green algae. London: Academic Press.
Hood, W., Leaver, A. G., Carr, N. G. 1969. Extracellular nitrogen and the control of arginine biosynthesis inAnabaena variabilis. Biochemical Journal144:12–13.
Kleiner, D. 1979. Regulation of ammonium uptake and metabolism by nitrogen-fixing bacteria. III.Clostridium pasteurianum. Archives of Microbiology120:263–270.
Lowry, O. H., Rosebrough, N. J., Farr, A., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry193:265–275.
Ohmori, M., Hattori, A. 1974. Effect of ammonia on nitrogen fixation by the blue-green algaAnabaena cylindrica. Plant and Cell Physiology15:131–142.
Pandey, D. C., Mitra, A. K. 1964. Free amino acid composition ofAnabaena naviculoides Fritsch at heterocystous and non-heterocystous stages. Die Naturwissenschaften51:248–249.
Puri, S., Grover, I. S. 1981. Nitrogen fixation and release of extracellular nitrogen byAnabaena G 102. Phykos20:99–101.
Roger, P. A., Kulasooriya, S. A. 1980. Blue-green algae and rice. Los Banos, Laguna, Philippines: International Rice Research Institute.
Rowell, P., Enticott, S., Stewart, W. D. P. 1977. Glutamine synthetase and nitrogenase activity in the blue-green algaAnabaena cylindrica. New Phytologist79:41–54.
Secor, J., Schrader, L. E. 1984. Characterization of amino acid efflux from isolated soybean cells. Plant Physiology74:26–31.
Singh, R. N. 1961. The role of blue-green algae in nitrogen economy of Indian agriculture. New Delhi: Indian Council of Agricultural Research.
Stewart, W. D. P. 1963. Liberation of extracellular nitrogen by two nitrogen-fixing blue-green algae. Nature (London)200:1020–1021.
Venkataraman, G. S. 1972. Algal biofertilizers and rice cultivation. New Delhi: Today and Tomorrow's Printers and Publications.
Watanabe, A. 1951. Production in cultural solution of some amino acids by the atmospheric nitrogen-fixing blue-green algae. Archives Biochemistry and Biophysics34:50–55.
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Antarikanonda, P. Production of extracellular free amino acids by cyanobacteriumAnabaena siamensis Antarikanonda. Current Microbiology 11, 191–195 (1984). https://doi.org/10.1007/BF01567159
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DOI: https://doi.org/10.1007/BF01567159