Abstract
Protein A coupled to alkaline phosphatase has been used for indirect enzyme-linked immunosorbent assay (ELISA) to identify strains ofRhizobium in culture and nodules. This conjugate shows no background reactions and provides a highly specific and sensitive detection of rhizobial antigens. An additional advantage is the simplicity of this technique for routine rhizobial detection. Currently, enzyme-labeled protein A has been used at considerably higher dilutions than conjugated goat anti-rabbit immunoglobulin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Ahmad, M. H., Eaglesham, A. R. J., Hassouna, S. 1981. Examining serological diversity of “Cowpea” rhizobia by the ELISA technique. Archives of Microbiology130:281–287.
Barbara, D. J., Clark, M. F. 1982. A simple indirect ELISA using F (ab′)2 fragments of immunoglobulin. Journal of General Virology58:315–322.
Berger, J. A., May, S. N., Berger, L. R., Bohlool, B. B. 1979. Colorimetric enzyme-linked immunosorbent assay for the identification of strains ofRhizobium in culture and in the nodules of lentils. Applied and Environmental Microbiology37:642–646.
Clark, M. F., Adams, A. N. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology34:475–483.
Engvall, E. 1978. Preparation of enzyme-labelled staphylococcal protein A and its use for detection of antibodies. Scandinavian Journal Immunology,8, Suppl. 7:25–31.
Engvall, E., Perlmann, P. 1971. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry8:871–874.
Hodgson, A. L. M., Waid, J. S. 1981. Use of enzyme-linked immunosorbent assay (ELISA) to identify bacteriocinogenic strains ofR. trifolii in nodules following a mixed strain inoculation, p. 340. In: Current Perspectives in Nitrogen Fixation, Proceedings of the Fourth International Symposium on Nitrogen Fixation (Canberra, Australia, 1–5 December, 1980).
Kishinevsky, B., Bar-Joseph, M. 1978. Rhizobium strains identification inArachis hypogaea nodules by enzymelinked immunosorbent assay (ELISA). Canadian Journal of Microbiology24:1537–1543.
Kishinevsky, B., Gurfel, D. 1980. Evaluation of enzymelinked immunosorbent assay (ELISA) for serological identification of differentRhizobium strains. Journal of Applied Bacteriology49:514–526.
Kronvall, G., Seal, U. S., Finstad, Y., Williams, R. C. 1970. Phylogenetic insight into evolution of mammalian Fc fragment of γG globulin using staphylococcal protein A. Journal of Immunology104:140–147.
Morley, S. J., Jones, D. G. 1980. A note on a highly sensitive modified ELISA technique forRhizobium strains identification. Journal of Applied Bacteriology49:103–109.
Olsen, P. E., Rice, W. A., Stemke, G., Page, W. J. 1981. Serological identification of Canadian selectedRhizobium meliloti strains in commercial inoculants, pp. 456–467. In: Proceedings of the 8th North AmericanRhizobium Conference, 3–7 September, 1981.
Vincent, J. M. 1970. A manual for the practical study of the root nodule bacteria. Blackwell Scientific Publications, Oxford, England.
Voller, A., Bidwell, D., Bartlett, A. 1976. Microplate enzyme immunoassays for the immunodiagnosis of virus infections. In: Rose, N. R., Friedman, H. (eds.), Manual of Clinical Immunology. Washington, D.C.: American Society of Microbiology.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kishinevsky, B., Maoz, A. ELISA identification ofRhizobium strains by use of enzymelabeled protein A. Current Microbiology 9, 45–49 (1983). https://doi.org/10.1007/BF01567132
Issue Date:
DOI: https://doi.org/10.1007/BF01567132