Abstract
The effect of serum supplementation was evaluated on 108 oocytes obtained from 29 patients. The oocytes were all mature and randomly distributed in two groups. Each ovum was paired with another ovum within the same patient. The experimental group contained no serum in the insemination and growth medium, but in the control group 10% serum was added to the insemination and growth medium. In the experimental group the cleavage rate was 66.7%, compared to 83.3% in the control group. The embryos from each patient were individually evaluated. The sign test was used for statistical analysis. A significant difference between the two groups (P=0.01) indicated that in this study the addition of serum to the medium gives better cleavage results. The effect of serum on embryos is discussed, as well as the practical implications of this finding.
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Marrs RP: Laboratory conditions for human in vitro fertilization procedures. Clin Obstet Gynecol 1986;29:180–189
Fishel SB, Edwards RG, Purdy JM: In vitro fertilization of human oocytes: Factors associated with embryonic development in vitro, replacement of embryos and pregnancy.In Fertilization of the Human Egg in Vitro, Beier, Lindner (eds). Berlin, Springer Verlag, 1983, pp 251–269
Van Zyl JA, Menkveld R, Kotze TJ, Van Niekerk WA: The importance of spermiograms that meet the requirements of international standards and the most important factors that influence semen parameters.In Proceedings of the 17th Congress of the International Society of Urology, Paris, 1976, p 263
Kruger TF, Van der Menve JP, Stander FSH,et al: The program and results of the In Vitro Fertilization Unit of the Tygerberg Hospital, University of Stellenbosch, Phase II and III. S Afr Med J 1986;69:297–300
Testart J, Frydman R, De Mouzon J, Lassale B, Belaisch JC: A study of factors affecting the success of human fertilization in vitro. I. Influence of ovarian stimulation upon the number and condition of oocytes collected. Biol Reprod 1983;28:415–424
Conver WJ: Practical Nonparametric Statistics. New York, Wiley, 1972, pp 121–130
Fishel SB, Surani MAH: Changes in responsiveness of preimplantation mouse embryos to serum. J Embryol Exp Morphol 1978;45:295–301
Menezo Y, Testart J, Perrone D: Serum is not necessary in human in vitro fertilization, early embryo culture and transfer. Fertil Steril 1984;42:750–755
Kruger TF, Stander FSH, Van der Merwe JP,et al: The technique for human embryo transfer at Tygerberg Hospital. Acta Eur Fertil 1986;17:117–119
Saito H, Berger T, Mishell DR, Marss RP: Effect of variable concentrations of serum on mouse embryo development. Fertil Steril 1984;41:460–464
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Kruger, T.F., Stander, F.S.H., Smith, K. et al. The effect of serum supplementation on the cleavage of human embryos. J Assist Reprod Genet 4, 10–12 (1987). https://doi.org/10.1007/BF01555428
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DOI: https://doi.org/10.1007/BF01555428