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Polyethylene-glycol-mediated cybrid formation: High-efficiency techniques and cybrid formation without enucleation

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Somatic Cell Genetics

Abstract

Polyethylene glycol (PEG) can be used to promote the fusion of enucleated cytoplasts from chloramphenicol (CAP)-resistant mouse cells with intact cells, resulting in the formation of viable cybrids. The techniques are simple and highly efficient, yielding up to one viable cybrid per 20 intact cells fused. It also seems that PEG can be used to induce cybrid formation without the necessity of prior enucleation of the CAP-resistant cells.

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Authors and Affiliations

  1. Division of Human Genetics, Children's Hospital Medical Center, 02115, Boston, Massachusetts

    Jay M. Wilson & Richard L. Davidson

  2. Sidney Farber Cancer Institute, 02115, Boston, Massachusetts

    Neil Howell & Ruth Sager

  3. Department of Microbiology and Molecular Genetics, Harvard Medical School, 02115, Boston, Massachusetts

    Neil Howell, Ruth Sager & Richard L. Davidson

  4. Department of Interdisciplinary Sciences, Massachusetts Institute of Technology, 02139, Cambridge, Massachusetts

    Jay M. Wilson

Authors
  1. Jay M. Wilson
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  2. Neil Howell
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  3. Ruth Sager
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  4. Richard L. Davidson
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Wilson, J.M., Howell, N., Sager, R. et al. Polyethylene-glycol-mediated cybrid formation: High-efficiency techniques and cybrid formation without enucleation. Somat Cell Mol Genet 4, 745–752 (1978). https://doi.org/10.1007/BF01543162

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  • DOI: https://doi.org/10.1007/BF01543162

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