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METT-1: A karyotypically normal in vitro line of developmentally totipotent mouse teratocarcinoma cells

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Somatic Cell Genetics

Abstract

A karyotypically normal, chromosomally female (X/X) in vitro line of mouse teratocarcinoma stem cells was established from a malignant mouse teratocarcinoma of the 129/Sv Sl C P inbred strain. The tumor of origin was experimentally induced by ectopic transplantation of a 6-day embryo. The normal number of chromosomes was observed in 92% of metaphases of the cultured cells. This high frequency of euploidy, as well as karyotypic normalcy, were maintained during numerous passages in culture without a feeder-cell layer and after freezing and thawing of the cells. The line has been designated METT-1 (Mouse Euploid Totipotent Teratocarcinoma), signifying that it is the first such in vitro line that has proved (in tests by T. Stewart and B. Mintz, manuscript in preparation) to be developmentally totipotent, i.e., capable of both somatic and germinal differentiation when injected into blastocysts, even after freezing and thawing and prolonged culture. This unique ensemble of properties renders the cell line suitable for selection of specific mutant genes and for gene-transfer experiments in culture, for the purpose of producing from the mutant cells new strains of mice with predetermined genetic changes.

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Literature cited

  1. Kleinsmith, L.J., and Pierce, G.B., Jr. (1964).Cancer Res. 24:1544–1551.

    Google Scholar 

  2. Mintz, B., Cronmiller, C., and Custer, R.P. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:2834–2838.

    Google Scholar 

  3. Diwan, S.B., and Stevens, L.C. (1976).J. Natl. Cancer Inst. 57:937–942.

    Google Scholar 

  4. Stevens, L.C., and Hummel, K.P. (1957).J. Natl. Cancer Inst. 18:719–747.

    Google Scholar 

  5. Mintz, B., and Illmensee, K. (1975).Proc. Natl. Acad. Sci. U.S.A. 72:3585–3589.

    Google Scholar 

  6. Illmensee, K., and Mintz, B. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:549–553.

    Google Scholar 

  7. Cronmiller, C., and Mintz, B. (1978).Dev. Biol. 67:465–477.

    Google Scholar 

  8. Papaioannou, V.E., McBurney, M.W., Gardner, R.L., and Evans, M.J. (1975).Nature 258:70–73.

    Google Scholar 

  9. Brinster, R.L. (1974).J. Exp. Med. 140:1049–1056.

    Google Scholar 

  10. McBurney, M.W. (1976).J. Cell Physiol. 89:441–456.

    Google Scholar 

  11. Mintz, B. (1977). InGenetic Interaction and Gene Transfer, Brookhaven Symposia in Biology, Vol. 29, (ed.) Anderson, C.W. (Brookhaven Natl. Labs., Upton, New York), pp. 82–95.

    Google Scholar 

  12. Mintz, B. (1978).Harvey Lect. 71:193–246.

    Google Scholar 

  13. Mintz, B. (1979).Differentiation 13:25–27.

    Google Scholar 

  14. Mintz, B. (1979). InModels for the Study of Inborn Errors of Metabolism, (ed.) Hommes, F.A. (Elsevier/North Holland, Amsterdam), pp. 343–354.

    Google Scholar 

  15. Dewey, M.J., Martin, D.W., Jr., Martin, G.R., and Mintz, B. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:5564–5568.

    Google Scholar 

  16. Watanabe, T., Dewey, M.J., and Mintz, B. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:5113–5117.

    Google Scholar 

  17. Pellicer, A., Wagner, E.F., El Kareh, A., Dewey, M.J., Reuser, A.J., Silverstein, S., Axel, R., and Mintz, B. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:2098–2102.

    Google Scholar 

  18. Stevens, L.C. (1970).Dev. Biol. 21:364–382.

    Google Scholar 

  19. Dunn, G.R., and Stevens, L.C. (1970).J. Natl. Cancer Inst. 44:99–105.

    Google Scholar 

  20. Solter, D., Dominis, M., and Damjanov, I. (1979).Int. J. Cancer 24:770–772.

    Google Scholar 

  21. Stevens, L.C. (1967).Adv. Morphogen. 6:1–31.

    Google Scholar 

  22. Stevens, L.C., and Varnum, D.S. (1974).Dev. Biol. 37:369–380.

    Google Scholar 

  23. Rizzino, A., and Sato, G. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:1844–1848.

    Google Scholar 

  24. Bottenstein, J.E., and Sato, G.H. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:514–517.

    Google Scholar 

  25. Oshima, R. (1978).Differentiation 11:149–155.

    Google Scholar 

  26. Chen, T.R. (1977).Exp. Cell Res. 104:255–262.

    Google Scholar 

  27. Klinger, H.P. (1972).Cytogenetics 11:424–435.

    Google Scholar 

  28. Stevens, L.C. (1960).Dev. Biol. 2:285–297.

    Google Scholar 

  29. Pierce, G.B., Jr., Dixon, F.J., Jr., and Verney, E.L. (1960).Lab. Invest. 9:583–602.

    Google Scholar 

  30. Nesbitt, M.N., and Francke, U. (1973).Chromosoma 41:145–158.

    Google Scholar 

  31. Cudennec, C.A., and Nicolas, J.-F. (1977).J. Embryol. Exp. Morphol. 38:203–210.

    Google Scholar 

  32. Finch, B.W., and Ephrussi, B. (1967).Proc. Natl. Acad. Sci. U.S.A. 57:615–621.

    Google Scholar 

  33. Rosenthal, M.D., Wishnow, R.M., and Sato, G.H. (1970).J. Natl. Cancer Inst. 44:1001–1009.

    Google Scholar 

  34. Lehman, J.M., Speers, W.C., Swartzendruber, D.E., and Pierce, G.B. (1974).J. Cell. Physiol. 84:13–27.

    Google Scholar 

  35. Martin, G.R., and Evans, M.J. (1975).Proc. Natl. Acad. Sci. U.S.A. 72:1441–1445.

    Google Scholar 

  36. Nicholas, J.F., Avner, P., Gaillard, J., Guénet, J.L., Jakob, H., and Jacob, F. (1976).Cancer Res. 36:4224–4231.

    Google Scholar 

  37. Martin, G.R., Epstein, C.J., Travis, B., Tucker, G., Yatziv, S., Martin, D.W., Jr., Clift, S., and Cohen, S. (1978).Nature 271:329–333.

    Google Scholar 

  38. Papaioannou, V.E., Gardner, R.L., McBurney, M.W., Babinet, C., and Evans, M.J. (1978).J. Embryol. Exp. Morphol. 44:93–104.

    Google Scholar 

  39. Gropp, A. (1975).Clin. Genet. 8:389.

    Google Scholar 

  40. Papaioannou, V.E., Evans, E.P., Gardner, R.L., and Graham, C.F. (1979).J. Embryol. Exp. Morphol. 54:277–295.

    Google Scholar 

  41. Papaioannou, V.E. (1979). InCell Lineage, Stem Cells and Cell Determination, INSERM Symposium, No. 10, (ed.) Le Douarin, N. (Elsevier/North Holland, Amsterdam), pp. 141–155.

    Google Scholar 

  42. Fellous, M., Günther, E., Kemler, R., Wiels, J., Berger, R., Guénet, J.L., Jakob, H., and Jacob, F. (1978).J. Exp. Med. 148:58–70.

    Google Scholar 

  43. McBurney, M.W., and Strutt, B.J. (1980).Cell 21:357–364.

    Google Scholar 

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Authors and Affiliations

  1. Institute for Cancer Research, Fox Chase Cancer Center, 19111, Philadelphia, Pennsylvania

    Beatrice Mintz & Claire Cronmiller

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  1. Beatrice Mintz
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  2. Claire Cronmiller
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Mintz, B., Cronmiller, C. METT-1: A karyotypically normal in vitro line of developmentally totipotent mouse teratocarcinoma cells. Somat Cell Mol Genet 7, 489–505 (1981). https://doi.org/10.1007/BF01542992

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