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Bromodeoxyuridine mutagenesis in mammalian cells is stimulated by purine deoxyribonucleosides

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Somatic Cell Genetics

Abstract

The effects of purine deoxyribonucleosides on bromodeoxyuridine (BrdU) mutagenesis in Syrian hamster melanoma cells were determined. Both deoxyguanosine (dG) and deoxyadenosine (dA) were found to stimulate mutagenesis without changing the amount of BrdU in DNA. In addition, the stimulation of mutagenesis by dG and dA was suppressed by the addition of deoxycytidine (dC). These results suggest that BrdU mutagenesis involves the perturbation of dC metabolism, which perturbation is enhanced by dGTP and dATP. The mutagenic activity of dG in the absence of BrdU was tested, as was that of thymidine (dT), which we had shown previously to stimulate BrdU mutagenesis. With dG alone, no increase above the spontaneous mutation frequency was detected. However, at extremely high concentration, dT in the absence of BrdU was slightly mutagenic, and the mutagenesis by dT was enhanced by dG and suppressed by dC.

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Authors and Affiliations

  1. Division of Human Genetics, Children's Hospital Medical Center, 02215, Boston, Massachusetts

    Elliot R. Kaufman & Richard L. Davidson

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, 02215, Boston, Massachusetts

    Elliot R. Kaufman & Richard L. Davidson

Authors
  1. Elliot R. Kaufman
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  2. Richard L. Davidson
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Kaufman, E.R., Davidson, R.L. Bromodeoxyuridine mutagenesis in mammalian cells is stimulated by purine deoxyribonucleosides. Somat Cell Mol Genet 5, 653–663 (1979). https://doi.org/10.1007/BF01542701

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  • DOI: https://doi.org/10.1007/BF01542701

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