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Polyethylene glycol-mediated fusion of human tumor cells with mouse cells

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Somatic Cell Genetics

Abstract

Polyethylene glycol (PEG)-mediated somatic cell fusion is shown to be dependent on temperature, the molecular weight of PEG used, and the fusion technique. Time-sequential formation of multinucleated cells is described. Cultures were established of hybrids obtained by PEG-mediated fusion of mouse cells and human tumor cells.

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Authors and Affiliations

  1. The Wistar Institute of Anatomy and Biology, 19104, Philadelphia, Pennsylvania

    Zenon Steplewski & Hilary Koprowski

  2. Scott and White Clinic, 76501, Temple, Texas

    Albert Leibovitz

Authors
  1. Zenon Steplewski
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  2. Hilary Koprowski
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  3. Albert Leibovitz
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Steplewski, Z., Koprowski, H. & Leibovitz, A. Polyethylene glycol-mediated fusion of human tumor cells with mouse cells. Somat Cell Mol Genet 2, 559–564 (1976). https://doi.org/10.1007/BF01542692

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  • DOI: https://doi.org/10.1007/BF01542692

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