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Parameters of polyethylene glycol-induced cell fusion and hybridization in lymphoid cell lines

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Somatic Cell Genetics

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Abstract

Methods employing polyethylene glycol (PEG) as a cell-fusion agent for mouse and human lymphoid cell lines were devised. Variables in these procedures were systematically explored. Under optimal conditions, PEG will induce 56% of the cells in the mouse cultures and 18% of the cells in the human cultures tested to undergo fusions resulting in multinucleated products. Intraspecific hybrids between mouse leukemic cells occur at a frequency of 26 × 10−5, and interspecific hybrids between mouse leukemic cells and Chinese hamster fibroblasts occur at 4 × 10−5. Differences in the requirements for maximum Sendai virus activity and PEG fusogenic activity are discussed.

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Authors and Affiliations

  1. Genetics Department, Iowa State University, 50011, Ames, Iowa

    V. L. Vaughan, D. Hansen & J. Stadler

Authors
  1. V. L. Vaughan
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  2. D. Hansen
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  3. J. Stadler
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Vaughan, V.L., Hansen, D. & Stadler, J. Parameters of polyethylene glycol-induced cell fusion and hybridization in lymphoid cell lines. Somat Cell Mol Genet 2, 537–544 (1976). https://doi.org/10.1007/BF01542690

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  • DOI: https://doi.org/10.1007/BF01542690

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