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IL-10 suppresses cell surface CD23/FcεRII expression, not by enhancing soluble CD23 release, but by reducing CD23 mRNA expression in human monocytes

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Abstract

To examine a possible involvement of interleukin-10 (IL-10) in CD23/FcεRII expression in human monocytes, effects of IL-10 on the cell surface CD23 expression, soluble CD23 (sCD23) release, and CD23 type b mRNA expression were investigated. IL-10 suppressed IL-4-induced surface CD23 expression on monocytes in a dose-dependent manner, and this effect was completely neutralized by anti-IL-10 antibody. The suppressive effect of IL-10 on surface CD23 expression was not due to enhancement of sCD23 release from the cell surface because no increase in sCD23 in culture supernatant was detected after incubation with IL-10. Instead, the effect of IL-10 seemed to be exerted at the transcriptional level since IL-4-induced expression of CD23 type b mRNA was significantly reduced when IL-10 was present. Although IL-4 induced surface CD23 expression on both monocytes and B cells, the suppressive effect of IL-10 was observed only on monocytes, which underscores different regulatory mechanisms for CD23 expression between the two cell types.

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Authors and Affiliations

  1. Department of Medicine and Clinical Science, Kyoto University, Graduate School of Medicine, 606, Kyoto, Japan

    Akio Morinobu, Hidetoshi Yanagida & Kazuwa Nakao

  2. Department of Laboratory Medicine, Kobe University Medical School, 650, Kobe, Japan

    Shunichi Kumagai

  3. Internal Medicine and Clinical Research Center, National Utano Hospital, 606, Kyoto, Japan

    Hiroyuki Ota & Hiroshi Ishida

  4. Department of Biological Responses, Virus Research Institute, Kyoto University, 606, Kyoto, Japan

    Minoru Matsui & Junji Yodoi

Authors
  1. Akio Morinobu
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  2. Shunichi Kumagai
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  3. Hidetoshi Yanagida
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  4. Hiroyuki Ota
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  6. Minoru Matsui
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  7. Junji Yodoi
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  8. Kazuwa Nakao
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Morinobu, A., Kumagai, S., Yanagida, H. et al. IL-10 suppresses cell surface CD23/FcεRII expression, not by enhancing soluble CD23 release, but by reducing CD23 mRNA expression in human monocytes. J Clin Immunol 16, 326–333 (1996). https://doi.org/10.1007/BF01541668

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  • DOI: https://doi.org/10.1007/BF01541668

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