Skip to main content
SpringerLink
Log in

Endoplasmic reticulum, calciosomes and their possible roles in signal transduction

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Recent fluorescence, AVEC-DIC, and confocal laser scanning microscopic studies have revealed the dynamic nature and structural extent of a calcium-sequestering endoplasmic reticulum (ER) in plant cells. Various investigators have proposed different roles for the ER in cell motility. One, the ER plays a direct role in the generation of intracellular particle motions or two, the ER regulates particle motions indirectly. We show that the ER can be extruded fromAcetabularia cells, stains brightly with the fluorescent dye DiOC6(3), and small (ca. 100 nm diameter) fluorescent vesicles are observed to move in or along the ER tubules. Intracellular particle movements in the giant algal cellAcetabularia can be transiently inhibited by IP4, IP3, and IP2, compounds which in animal cells are known to cause the release of free calcium ions. A model is proposed which clarifies the possible relationships between the ER, calciosomes, calcium ions, and the microfilament-generated intracellular particle movements observed in plant cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AVEC-DIC:

video microscopy in differential interference contrast

CFLSM:

confocal laser scanning microscope

DiOC6(3):

3,3′-dihexyloxacarbocyanine iodide

ER:

endoplasmic reticulum

IP3 :

inositol triphosphate

N.A.:

numerical aperture

SIT:

silicon intensified target video camera

SR:

sarcoplasmic reticulum

References

  • Allen NS (1980) Cytoplasmic streaming and transport in the characean algaNitella. Can J Bot 58: 786–796

    Google Scholar 

  • —, Brown DT (1988) Dynamics of the endoplasmic reticulum in living onion epidermal cells in relation to microtubules, microfilaments, and intracellular particle movement. Cell Motil Cytoskeleton 10: 153–163

    Google Scholar 

  • —, O'Connor SA (1990) Inhibition of intracellular particle motions inAcetabularia acetabulum L. by phosphoinositides. In: Morré DJ, Boss WF, Loewus FA (eds) Plant inositides. Alan R Liss, New York, pp 301–310

    Google Scholar 

  • Allen RD, Allen NS, Travis J (1981) Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: a new method capable of analyzing microtubule-related motility in the reticulopodial network ofAllegromia laticollaris. Cell Motil 1: 291–302

    PubMed  Google Scholar 

  • Berridge MJ (1987) Inositol trisphosphate and diacylglycerol: two interacting second messengers. Annu Rev Biochem 56: 159–194

    PubMed  Google Scholar 

  • Chou MK, Krause K, Campbell K, Jensen K, Sjolund R (1989) Calsequestrin: a novel calcium binding protein in plants. Plant Physiol 89: 149

    Google Scholar 

  • Fliegel L, Ohnishi M, Carpenter MR, Khanna VK, Reithmeier RAF, McLennan DH (1987) Amino acid sequence of rabbit fast-twitch skeletal muscle calsequestrin deduced from cDNA and peptide sequencing. Proc Natl Acad Sci USA 84: 1167–1171

    PubMed  Google Scholar 

  • Hashimoto S, Bruno B, Lew DP, Pozzan T, Volpe P, Meldolesi J (1988) Immunocytochemistry of calciosomes in liver and pancreas. J Cell Biol 107: 2523–2531

    PubMed  Google Scholar 

  • Hayama T, Shimmen T, Tazawa M (1979) Partizipation of Ca++ in cessation of cytoplasmic streaming induced by membrane excitation in characeae internodal cells. Protoplasma 99: 305–321

    Google Scholar 

  • Kachar B, Reese TS (1988) The mechanism of cytoplasmic streaming in characean algal cells: sliding of endoplasmic reticulum along actin filaments. J Cell Biol 106: 1545–1552

    PubMed  Google Scholar 

  • Krause KM, Chou M, Thomas MA, Sjolund RD (1989) Plant cells contain calsequestrin. J Biol Chem 264: 4269–4272

    PubMed  Google Scholar 

  • Marmé D (1989) The role of calcium and calmodulin in signal transduction. In: Boss WF, Morré DJ (eds) Second messengers in plant growth and development. Alan R Liss, New York, pp 57–80

    Google Scholar 

  • Nagai R, Hayama T (1979) Ultrastructure of the endoplasmic factor responsible for cytoplasmic streaming inChara internodal cells. J Cell Sci 36: 121–136

    PubMed  Google Scholar 

  • Oberdorf JA, Head JF, Kaminer B (1986) Calcium uptake and release by isolated cortices and microsomes from the unfertilized egg of the sea urchinStrongylocentrotus droebachiensis. J Cell Biol 102: 2205–2210

    PubMed  Google Scholar 

  • —, Lebeche D, Head JF, Kaminer B (1988) Identification of a Calsequestrin-like protein from sea urchin eggs. J Biol Chem 263: 6806–6809

    PubMed  Google Scholar 

  • Palade GE, Porter KR (1954) Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med 100: 541–656

    PubMed  Google Scholar 

  • Quader H, Hoffmann A, Schnepf E (1987) Shape and movement of the endoplasmic reticulum in onion bulb epidermis cells: possible involvement of actin. Eur J Cell Biol 44: 17–26

    Google Scholar 

  • Scott BT, Simmerman HKB, Collins JH, Nadal-Ginard B, Jones LR (1988) Complete amino acid sequence of canine cardiac calsequestrin deduced by cDNA cloning. J Biol Chem 263: 8958–8964

    PubMed  Google Scholar 

  • Sheetz MP, Spudich JP (1983) Movement of myosin-coated fluorescent beads on actin cables in vitro. Nature 303: 31–35

    PubMed  Google Scholar 

  • Teresaki M, Serdat C (1989) Localization of calcium uptake and release to sea urchin egg cortical ER using the calcium indicator dye Fluo-3. J Cell Biol 109: 302 a

    Google Scholar 

  • —, Chen LV, Fujiwara K (1986) Microtubules and the endoplasmic reticulum are highly interdependent structures. J Cell Biol 103: 1557–1568

    PubMed  Google Scholar 

  • Volpe P, Krause KH, Hashimoto S, Zorzato F, Pozzan T, Meldolesi J, Lew DP (1988) “Calciosome”, a cytoplasmic organelle: the inositol-l,4,5-triphosphate-sensitive Ca++ store of nonmuscle cells? Proc Natl Acad Sci USA 85: 1091–1095

    PubMed  Google Scholar 

  • Williamson RE (1979) Filaments associated with the endoplasmic reticulum in the streaming cytoplasm ofChara corallina. Eur J Cell Biol 20: 177–183

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Wake Forest University, Winston-Salem, North Carolina

    Nina Strömgren Allen & Joan H. Schumm

Authors
  1. Nina Strömgren Allen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joan H. Schumm
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Strömgren Allen, N., Schumm, J.H. Endoplasmic reticulum, calciosomes and their possible roles in signal transduction. Protoplasma 154, 172–178 (1990). https://doi.org/10.1007/BF01539845

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01539845

Keywords

Search

Navigation