Abstract
We have examined the restriction endonuclease cleavage patterns exhibited by the mitochondrial DNAs (mtDNA) of four chloramphenicolresistant (CAPR)human × mouse hybrids and one CAPR cybrid derived from CAPR HeLa cells and CAPS mouse RAG cells. Restriction fragments of mtDNAs were separated by electrophoresis and transferred by the Southern technique to diazobenzyloxymethyl paper. The covalently bound DNA fragments were hybridized initially with 32 P-labeled complementary RNA (cRNA) prepared from human mtDNA and, after removal of the human probe, hybridized with mouse [32P]cRNA prepared from mouse mtDNA. Three hybrids which preferentially segregated human chromosomes and the cybrid exhibited mtDNA fragments indistinguishable from mouse cells. One hybrid, ROH8A, which exhibited “reverse” chromosome segregation, contained only human mtDNA. The pattern of chromosome and mtDNA segregation observed in these hybrids and the cybrid support the hypothesis that a complete set of human chromosomes must be retained if a human mouse hybrid is to retain human mitochondrial DNA.
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Literature cited
Gillham, N.W. (1978).Organelle Heredity, (Raven Press, New York).
Angerer, L., Davidson, N., Murphy, W., Lynch, D., and Attardi, G. (1976).Cell 9:81–90.
Ohi, S., Ramirez, J.L., Upholt, W.B., and Dawid, I.B. (1978).J. Mol. Biol. 121:299–310.
Coon, H.G., Horak, I., and Dawid, I.B. (1973).J. Mol. Biol. 81:285–298.
Jakovcic, S., Casey, J., and Rabinowitz, M. (1975).Biochemistry 14:2043–2050.
Upholt, W.B., and Dawid, I.B. (1977).Cell 11:571–583.
Brown, W.M., George, M., Jr., and Wilson, A.C. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:1967–1971.
Spolsky, C.M., and Eisenstadt, J.M. (1972).FEBS Lett. 25:319–324.
Bunn, C.L., Wallace, D.C., and Eisenstadt, J.M. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:1681–1685.
Wallace, D.C., Bunn, C.L., and Eisenstadt, J.M. (1975).J. Cell Biol. 67:174–188.
Wallace, D.C., and Eisenstadt, J.M. (1979).Somat. Cell Genet. 5:373–396.
Klebe, R.J., Chen, T.-R., and Ruddle, F.H. (1970).J. Cell Biol. 45:74–82.
Wahl, G.M., Stern, M., and Stark, G.R. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:3683–3687.
Southern, E.M. (1975).J. Mol. Biol. 98:503–517.
Bogenhagen, D., and Clayton, D.A. (1974).J. Biol. Chem. 249:7991–7995.
Burgess, R.R. (1969).J. Biol. Chem. 244:6160–6167.
Ehrlich, S.D., Bursztyn-Pettegrew, H., Stroynowski, I., and Lederberg, J. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:4145–4149.
Parker, R.C., and Watson, R.M. (1977).Nucleic Acids Res. 4:1291–1299.
Brown, W.M., and Goodman, H.M. (1979). InExtrachromosomal DNA, (eds.) Cummings, D.J., Borst, P., Dawid, I.B., Weissman, S.M., and Fox, C.F. (Academic Press, New York), pp. 485–499.
Moore, K.H., Johnson, P.H., Chandler, S.E.W., and Grossman, L.I. (1977).Nucleic Acids Res. 4:1273–1289.
Battey, J., and Clayton, D.A. (1978).Cell 14:143–156.
Ojala, D., and Attardi, G. (1977).Plasmid 1:78–105.
Wallace, D.C., Pollack, Y., Bunn, C.L., and Eisenstadt, J.M. (1976).In Vitro 12:758–776.
Clayton, D.A., Teplitz, R.L., Nabholz, M., Dovey, H., and Bodmer, W. (1971).Nature 234:560–562.
Attardi, B., and Attardi, G. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:129–133.
Minna, J.D., and Coon, H.G. (1974).Nature 252:401–404.
Jami, J., and Grandchamp, S. (1971).Proc. Natl. Acad. Sci. U.S.A. 68:3097–3101.
Croce, C.M. (1976).Proc. Natl. Acad. Sci. U.S.A. 73:3248–3252.
Creagan, R.P., and Ruddle, F.H. (1977). InMolecular Structure of Human Chromosomes, (ed.) Yunis, J.J. (Academic Press, New York), pp. 89–142.
Borst, P., and Grivell, L.A. (1978).Cell 15:705–723.
Linnane, A.W., and Nagley, P. (1978).Plasmid 1:324–345.
Smith, C.A., and Vinograd, J. (1972).J. Mol. Biol. 69:163–178.
DeLap, R.J., Rush, M.G., Zouzias, D., and Kahn, S. (1978).Plasmid 1:508–521.
Kuo, M.T., Meinke, W., and Sanders, G.F. (1975).Proc. Natl. Acad. Sci. U.S.A. 72:5004–5006.
Perry, R.P., Kelley, D.E., Schibler, U., Huebner, K., and Croce, C.M. (1979).J. Cell. Physiol. 98:553–560.
Marshall, C.J., Handmaker, S.D., and Bramwell, M.E. (1975).J. Cell Sci. 17:307–325.
Ajiro, K., Zweidler, A., Borum, T., and Croce, C.M. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:5599–5603.
Wallace, D.C., Bunn, C.L., and Eisenstadt, J.M. (1977).Somat. Cell Genet. 3:93–119.
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Giles, R.E., Stroynowski, I. & Wallace, D.C. Characterization of mitochondrial DNA in chloramphenicol-resistant interspecific hybrids and a cybrid. Somat Cell Mol Genet 6, 543–554 (1980). https://doi.org/10.1007/BF01539155
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DOI: https://doi.org/10.1007/BF01539155