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Somatic cell genetic analysis of HLA-A, B, C and humanβ 2-microglobulin expression

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Somatic Cell Genetics

Abstract

We have examined the cell surface expression of the human histocompatibility antigens HLA-A, B, C and β2-microglobulin (β2m) on a human-mouse somatic cell hybrid line. Using specific antibodies and the fluorescence-activated cell sorter (FACS), we viably fractionated and characterized four separate hybrid subpopulations (HLA+,β2m+; HLA +,β2m−; HLA−,β2m+; HLA−,β2m−). Hybrid selection based on surface antigen expression resulted in corresponding genetic selection for and against human chromosomes 6 and 15. Studies of the homogeneous hybrid sublines revealed that the presence of human β2m in a hybrid cell dramatically increased the surface expression of human HLA-A, B, C and mouse H-2Kk antigens. The results demonstrate the importance of human chromosome-specific surface markers and the fluorescence-activated cell sorter in somatic cell genetic analysis.

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Authors and Affiliations

  1. Departments of Biology and Human Genetics, Yale University, 06511, New Haven, Connecticut

    Michael E. Kamarck, James A. Barbosa & Frank H. Ruddle

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  1. Michael E. Kamarck
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  2. James A. Barbosa
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  3. Frank H. Ruddle
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Kamarck, M.E., Barbosa, J.A. & Ruddle, F.H. Somatic cell genetic analysis of HLA-A, B, C and humanβ 2-microglobulin expression. Somat Cell Mol Genet 8, 385–402 (1982). https://doi.org/10.1007/BF01538895

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  • DOI: https://doi.org/10.1007/BF01538895

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