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Expression of cytoplasmically inherited genes for chloramphenicol resistance in interspecific somatic cell hybrids and cybrids

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Somatic Cell Genetics

Abstract

Interspecies cybrids and hybrids were prepared in which one parent contributed the cytoplasmically inherited mutation for mitochondrial chloramphenicol (CAP) resistance. The transfer of CAP-resistant (R) mitochondria in human × mouse cybrids proved to be extremely difficult. Transfer from mouse to hamster cells occurred, but the cybrids had limited proliferative capacity. Hybrids between CAP-R mouse and CAP-sensitive (S) hamster cells could be selected in HAT + CAP at a very low frequency but comparable hybrids could not be isolated in CAP-R human × CAP-S mouse fusions unless ouabain was substituted for HAT. Hybrids selected directly in CAP retained the chromosomes of the resistant parent and segregated those of the sensitive. CAP-R hybrids (CAP-R human × CAP-S mouse) which segregate human chromosomes could be isolated only by sequential selection in HAT and then CAP. These results suggest that the mitochondrial genes of mammalian species are rapidly diverging from each other.

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Authors and Affiliations

  1. Department of Genetics, Stanford University School of Medicine, 94305, Stanford, California

    Douglas C. Wallace

  2. Department of Human Genetics, Yale University, 333 Cedar Street, 06510, New Haven, Connecticut

    Jerome M. Eisenstadt

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  1. Douglas C. Wallace
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  2. Jerome M. Eisenstadt
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Wallace, D.C., Eisenstadt, J.M. Expression of cytoplasmically inherited genes for chloramphenicol resistance in interspecific somatic cell hybrids and cybrids. Somat Cell Mol Genet 5, 373–396 (1979). https://doi.org/10.1007/BF01538849

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  • DOI: https://doi.org/10.1007/BF01538849

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