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Restriction enzyme analysis of American region dengue viruses

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Summary

Restriction fragment heterogeneity of Hae III digestion products of cDNA to virion RNA was used to map the distribution of dengue virus topotypes found in the American region. By comparing the electrophoretic patterns of fragments produced, dengue virus isolates were placed in groups that agreed with those previously determined by oligonucleotide fingerprinting. Dengue-1 and dengue-4 viruses occur throughout the western hemisphere as single genetic types, with most of the isolates sharing at least 70% of their Hae III restriction enzyme fragments. Dengue-2 virus exists as two topotypes in the region with apparently non-overlapping distributions. The Puerto Rico topotype, which has been in the Caribbean for at least 40 years, is genetically diverse, while the Jamaica topotype, first isolated in 1981, is more homogeneous and has expanded its range from the original Caribbean focus to South America.

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References

  1. Anderson CR, Downs WG, Hill AE (1956) Isolation of dengue virus from a human being in Trinidad. Science 124: 224–225

    PubMed  Google Scholar 

  2. Blok J, Kay BH, Hall RA, Gorman BM (1988) Isolation and characterization of dengue viruses serotype 1 from an epidemic in northern Queensland, Australia. Arch Virol 100: 213–220

    PubMed  Google Scholar 

  3. Bres P (1979) Dengue in the Caribbean, 1977. Proc Workshop in Montego Bay, Jamaica, May 8–11, 1978, pp 4–10 (Pan American Health Organisation Publication, vol. 375)

  4. Burke DS, Nisalak A, Johnson DE, Scott RM (1988) A prospective study of dengue infections in Bankok. Am J Trop Med Hyg 38: 172–180

    PubMed  Google Scholar 

  5. Faragher SG, Hutchison CA, Dalgarno L (1985a) Analysis of Ross River virus genomic RNA using Hae III digests of single-stranded cDNA to infected-cell RNA and virion RNA. Virology 141: 248–256

    PubMed  Google Scholar 

  6. Faragher SG, Marshall ID, Dalgarno L (1985b) Ross River virus genetic variants in Australia and the Pacific islands. Aust J Exp Biol Med Sci 63: 473–488

    PubMed  Google Scholar 

  7. Gubler DJ (1987) Dengue and dengue hemorrhagic fever in the Americas. Puerto Rico Health Sci J 6: 107–111

    Google Scholar 

  8. Gubler DJ (1988) Dengue. In: Monath TP (ed) The arboviruses: biology and epidemiology. CRC Press, Boca Raton, pp 223–258

    Google Scholar 

  9. Gubler DJ (1989) Surveillance for dengue and dengue hemorrhagic fever. Bull Pan Amer Health Organ 23: 397–404

    Google Scholar 

  10. Halstead SB (1980) Dengue haemorrhagic fever-a public health problem and a field for research. Bull World Health Organ 58: 1–21

    Google Scholar 

  11. Henchal EA, McCown JM, Seguin MC, Gentry MK, Brandt WE (1986) Identification of an antigenic and genetic variant of dengue 4 virus from the Caribbean. Am J Trop Med Hyg 35: 393–400

    PubMed  Google Scholar 

  12. Kliks SC, Nimmanitya S, Nisalak A, Burke DS (1988) Evidence that maternal dengue antibodies are important in the development of dengue hemorrhagic fever in infants. Am J Trop Med Hyg 38: 411–419

    PubMed  Google Scholar 

  13. Lewis JA, Chang G-J, Lanciotti RS, Kinney RM, Mayer LW, Trent DW (1993) Phylogenetic relationships of dengue-2 viruses. Virology 197: 216–224

    PubMed  Google Scholar 

  14. Lobigs M, Weir RC, Dalgarno L (1986a) Genetic analysis of kunjin virus isolates using Hae III and Taq1 restriction digests of single-stranded cDNA to virion RNA. Aust J Exp Biol Med Sci 64: 185–196

    PubMed  Google Scholar 

  15. Lobigs M, Marshall ID, Weir HC, Dalgarno L (1986b) Genetic differentiation of Murray Valley encephalitis virus in Australia and Papua New Guinea. Aust J Exp Biol Med Sci 64: 571–585

    PubMed  Google Scholar 

  16. Mathiot CC, Georges AJ, Deubel V (1990) Comparative analysis of West Nile virus strains isolated from human and animal hosts using monoclonal antibodies and cDNA restriction digest profiles. Res Virol 141: 533–543

    PubMed  Google Scholar 

  17. Molloy PL, Symons RH (1980) Cleavage of DNA. RNA hybrids by type II restriction enzymes. Nucleic Acids Res 8: 2939–2946

    PubMed  Google Scholar 

  18. Monath TP, Kinney RM, Schlesinger JJ (1983) Ontogeny of yellow fever 17D vaccine: RNA oligonucleotide fingerprint and monoclonal antibody analyses of vaccines produced world-wide. J Gen Virol 64: 627–637

    PubMed  Google Scholar 

  19. Monath TP, Wands JR, Hill LJ, Brown NV, Marciniak RA, Wong MA, Gentry MK, Burke DS, Grant JA, Trent DW (1986) Geographic classification of dengue-2 virus stains by antigen signature analysis. Virology 154: 313–324

    PubMed  Google Scholar 

  20. Rico-Hesse R (1990) Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology 174: 479–493

    PubMed  Google Scholar 

  21. Schlesinger RW (1979) Dengue viruses. Springer, Wien New York (Virology Monographs, vol. 6)

    Google Scholar 

  22. Sudhanshu V, Faragher SG, Weir RC, Dalgarno L (1986) Ross River virus mutant with a deletion in the E2 gene: properties of the virion, virus-specific macromolecule synthesis, and attenuation of virulence for mice. Virology 151: 222–232

    PubMed  Google Scholar 

  23. Trent DW, Grant JA, Vorndam AV, Monath TP (1981) Genetic heterogeneity among Saint Louis encephalitis virus isolates of different geographic origin. Virology 114: 319–332

    PubMed  Google Scholar 

  24. Trent DW, Grant JA, Rosen L, Monath TP (1983) Genetic variation among dengue 2 viruses of different geographic origin. Virology 128: 271–284

    PubMed  Google Scholar 

  25. Trent DW, Manske CL, Fox GE, Chu MC, Kliks SC, Monath TP (1990) The molecular epidemiology of dengue viruses. Genetic variation and microevolution. Appl Virol Res 2: 293–315

    Google Scholar 

  26. Vezza A, Rosen L, Repik P, Dalrymple JM, Russell PK (1980) Characterization of the viral RNA species of prototype dengue viruses. Am J Trop Med Hyg 29: 643–652

    PubMed  Google Scholar 

  27. Walker PJ, Henchal EA, Blok J, Repik PM, Henchal LS, Burke DS, Robbins SJ, Gorman BM (1988) Variation in dengue type 2 viruses isolated in Bangkok during 1980. J Gen Virol 69: 591–602

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Vector-Borne Infectious Diseases, Dengue Laboratory, Centers for Disease Control and Prevention, San Juan, Puerto Rico

    V. Vorndam

  2. Department of Virology, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

    R. M. R. Nogueira

  3. Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

    D. W. Trent

Authors
  1. V. Vorndam
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  2. R. M. R. Nogueira
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  3. D. W. Trent
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Vorndam, V., Nogueira, R.M.R. & Trent, D.W. Restriction enzyme analysis of American region dengue viruses. Archives of Virology 136, 191–196 (1994). https://doi.org/10.1007/BF01538828

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  • DOI: https://doi.org/10.1007/BF01538828

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