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Identification of a mouse gene required for binding of Rauscher MuLV envelope gp70

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Somatic Cell Genetics

Abstract

Mouse chromosome segregating somatic cell hybrids were established between a mouse thymic leukemia cell line (GRSL) and Chinese hamster E36 cells. The GRSL cells specifically bound purified Rauscher leukemia virus gp70 while the E36 cells exhibited no binding. The hybrids selectively bound Rauscher gp70 depending on the presence of a mouse cellular gene for the ecotropic murine leukemia gp70 receptor. A syntenic relationship was observed between the DIP-3 chromosome marker (on chromosome 5) and the gp70 receptor in primary clones and subclones of these hybrids; this was confirmed by chromosome analysis. The involvement of H-2 in the binding of Rauscher MuLV gp70 could be ruled out, because discordancies of the receptor presence and H-2 absence as well as of the receptor absence and H-2 presence type could be observed. Our results indicate that the Rec-1(replication ecotropic MuLV) gene of Gazdar et al. (4) may well be the receptor gene for the ecotropic murine leukemia virus.

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Authors and Affiliations

  1. Division of Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066, CX, Amsterdam, The Netherlands

    John Hilkens, Alfonso Colombatti & Jo Hilgers

  2. Department of Pharmacologic and Experimental Therapeutics, Johns Hopkins University, Baltimore, Maryland

    Mette Strand

  3. Department of Biology, Yale University, 06520, New Haven, Connecticut

    Elisabeth Nichols

Authors
  1. John Hilkens
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  2. Alfonso Colombatti
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  3. Mette Strand
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  4. Elisabeth Nichols
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  5. Frank H. Ruddle
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  6. Jo Hilgers
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Hilkens, J., Colombatti, A., Strand, M. et al. Identification of a mouse gene required for binding of Rauscher MuLV envelope gp70. Somat Cell Mol Genet 5, 39–49 (1979). https://doi.org/10.1007/BF01538785

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  • DOI: https://doi.org/10.1007/BF01538785

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