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Replication of human chromosomes in human-mouse hybrids: Evidence that the timing of DNA synthesis is determined independently in each human chromosome

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Somatic Cell Genetics

Abstract

The terminal phase of DNA replication was studied by autoradiography in hybrids between human lymphocytes and mouse fibroblasts. The hybrids contained on the average only 11 human chromosomes. It was found that the sequence of terminal DNA replication for the human chromosomes in the hybrids was the same as the sequence of terminal replication for the corresponding chromosomes in the human lymphocytes. Furthermore, it was shown that the maintenance of the normal terminal replication sequence of the human chromosomes in the hybrids was not dependent on the presence of any specific human chromosome. The results suggest that the timing of terminal DNA replication is determined independently in each human chromosome.

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Literature cited

  1. Gilbert, C. W., Muldal, S., Lajtha, L. G., and Rowley, J. (1962).Nature 195:869–873.

    PubMed  Google Scholar 

  2. German, J. (1964).J. Cell Biol. 20:37–55.

    PubMed  Google Scholar 

  3. Gavosto, F., Pegoraro, L., Masera, P., and Rovera, G. (1968).Exp. Cell. Res. 49:340–358.

    PubMed  Google Scholar 

  4. Takagi, N., and Sandberg, A. A. (1968).Cytogenetics 7:135–143.

    PubMed  Google Scholar 

  5. Weiss, M., and Green, H. (1967).Proc. Natl. Acad. Sci. 58:1104–1111.

    PubMed  Google Scholar 

  6. Littlefield, J. (1964).Science 145:709.

    PubMed  Google Scholar 

  7. Davidson, R. L., and Ephrussi, B. (1965).Nature 205:1170–1171.

    Google Scholar 

  8. Caspersson, T., Zech, L., Johansson, C., and Modest, E. J. (1970).Chromosoma 30:215–227.

    PubMed  Google Scholar 

  9. Gallimore, P. H., and Richardson, C. R. (1973).Chromosoma 41:259–263.

    PubMed  Google Scholar 

  10. 1971 Paris Conference (1972).Standardization in Human Cytogenetics, Birth Defects: Original Article Series, Vol. VIII, The National Foundation, New York, p. 7.

  11. Rao, P. N., and Johnson, R. T. (1971).Nature 225:159–164.

    Google Scholar 

  12. Slezinger, S. I., and Prokofieva-Belgovskaya, A. A. (1968).Cytogenetics 7:337–346.

    PubMed  Google Scholar 

  13. German, J., and Aronian, D. (1971).Chromosoma 35:99–110.

    PubMed  Google Scholar 

  14. Sonnenschein, C. (1970).Exp. Cell Res. 63:195–199.

    PubMed  Google Scholar 

  15. Graves, J. A. M. (1972).Exp. Cell Res. 73:81–94.

    PubMed  Google Scholar 

  16. Marin, G., and Colletta, G. (1975).Exp. Cell Res., in press.

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Authors and Affiliations

  1. Division of Human Genetics, Children's Hospital Medical Center, USA

    M. S. Lin & R. L. Davidson

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts

    M. S. Lin & R. L. Davidson

Authors
  1. M. S. Lin
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  2. R. L. Davidson
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Lin, M.S., Davidson, R.L. Replication of human chromosomes in human-mouse hybrids: Evidence that the timing of DNA synthesis is determined independently in each human chromosome. Somat Cell Mol Genet 1, 111–122 (1975). https://doi.org/10.1007/BF01538735

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  • DOI: https://doi.org/10.1007/BF01538735

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