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Lipoprotein pattern and plasma lipoprotein lipase activities in patients with primary biliary cirrhosis

Relationship with increase of HDL2 fraction in Lp-X-positive and Lp-X-Negative subjects

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Abstract

Plasma lipids, apoprotein A-I and B in serum and in lipoprotein fractions (VLDL + LDL, HDL2, and HDL3) obtained by preparative ultracentrifugation, as well as postheparin lipoprotein lipase activity (H-TGL and LPL) were evaluated in 17 subjects with primary biliary cirrhosis (stage II and III) subdivided into two groups according to the presence or absence of lipoprotein X (Lp-X). A reduction in total lipoprotein lipase activity was observed in both patient groups, compared to controls (P<0.01); the hepatic lipoprotein lipase was significantly reduced (P<0.01) only in the Lp-X-positive group. The lipid (477.8+154.3 vs 239.6±51.1; P<0.01) and protein (147.4±37.1 vs 83.3±19.7; P< 0.01) masses in the VLDL + LDL fraction of the Lp-X-positive group were increased compared to controls. In the same group, the HDL2 fraction also showed an increase in lipid (186.6±80.0 vs 77.9±21.6; P<0.01) and protein (133.9±60.0 vs 67.9±16.5; P <0.01) masses; in addition, the HDL2 percent lipid composition was different in the two patient groups, showing a decrease in esterified cholesterol (20.4±3.6 vs 25.7±2.2; P <0.01) and an increase in phospholipids (59.2±2.9 vs 54.8±2.6; p<0.01) in the Lp-X-positive group. Apo B was also increased in Lp-X-positive patients both in the serum (134.0±27.6 vs 90.9±7.3; P<0.01) and in the VLDL + LDL fraction (134.0±22.2 vs 72.5±16.5; P<0.01). The differences seen in lipoprotein concentration and composition in the two patient groups seem related, in part, to the presence of Lp-X or, better, to the stage of the disease. The reduction in hepatic lipase may play an important role in determining the increase and altered composition of the HDL2 subfraction.

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References

  1. James SP (moderator): Primary biliary cirrhosis: A model autoimmune disease. Ann Intern Med 99:500–512, 1983

    PubMed  Google Scholar 

  2. Seidel D, Alaupovic P, Furman RH, McConathy WJ: A lipoprotein characterizing obstructive jaundice. II Isolation and partial characterization of the protein moieties of low density lipoproteins. J Clin Invest 59:2396–2407, 1970

    Google Scholar 

  3. Manzato E, Fellin R, Baggio G, Walch S, Neubeck W, Seidel D: Formation of lipoprotein-X; its relationship to bile compounds. J Clin Invest 57(5): 1248–1260, 1976

    PubMed  Google Scholar 

  4. Sabesin SM: Cholestatic lipoproteins. Their pathogenesis and significance. Gastroenterology 83(3):704–708, 1982

    PubMed  Google Scholar 

  5. Ahrens EH Jr, Kunkel HG: The relationship between serum lipids and skin xanthomata in eighteen patients with primary biliary cirrhosis. J Clin Invest 28:1565–1574, 1949

    PubMed  Google Scholar 

  6. Baruch Y, Brook JR, Eidelman S, Aviram M: Increased concentration of high density lipoprotein in plasma and decreased platelet aggregation in primary biliary cirrhosis. Atherosclerosis 53:151–162, 1984

    PubMed  Google Scholar 

  7. Aly A, Carlson K, Johansson C, Kirstein P, Rössner S, Wallentin L: Lipoprotein abnormalities in patients with early primary biliary cirrhosis. Eur J Clin Invest 14:156–162, 1984

    Google Scholar 

  8. Jahn CE, Schaefer EJ, Taam LA, Hoofnagle JH, Lindgren FT, Albers JJ, Jones EA, Brewer HB Jr: Lipoprotein abnormalities in primary biliary cirrhosis. Association with hepatic lipase inhibition as well as altered cholesterol esterification. Gastroenterology 89:1266–1278, 1985

    PubMed  Google Scholar 

  9. Koga S, Miyata Y, Ibayashi H: Plasma lipoproteins and apoproteins in primary biliary cirrhosis. Hepatology 5(2):286–292, 1985

    PubMed  Google Scholar 

  10. Blomhoff JP: High density lipoproteins in cholestasis. Scand J Gastroenterol 9:591–596, 1974

    PubMed  Google Scholar 

  11. Mc Intyre N, Harry DS, Pearson AJG: The hypercholesterolaemia of obstructive jaundice. Gut 16:379–391, 1975

    PubMed  Google Scholar 

  12. Agorastos J, Fox C, Harry DS, Mc Intyre N: Lecithin-cholesterol acyl-transferase and lipoprotein abnormalities of obstructive jaundice. Clin Sci Mol Med 54:369–379, 1978

    PubMed  Google Scholar 

  13. Blomhoff JP, Skrede S, Ritland S: Lecithin:cholesterol acyltransferase and plasma proteins in liver disease. Clin Chim Acta 53:197–207, 1974

    PubMed  Google Scholar 

  14. Danielsson B, Ekman R, Petersson BG: An abnormal high density lipoprotein in cholestatic plasma isolated by zonal ultracentrifugation. FEBS Lett 50(2): 180–184, 1975

    PubMed  Google Scholar 

  15. Etienne J, Beucler I, Ayrault M, Cloarec M, Polonovski J, Debray J: Abnormal lipoproteins in a case of primary biliary cirrhosis. Biomedicine 29:33–37, 1978

    PubMed  Google Scholar 

  16. Scheuer PJ: Primary Biliary cirrhosis. Proc R Soc Med 60:1257–1260, 1967

    PubMed  Google Scholar 

  17. Burstein M, Scholnick HR, Morfin R: Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res 11:583–595, 1970

    PubMed  Google Scholar 

  18. Wieland H, Seidel D: Eine neue und vereinfachte Methode zum Nachweis des Lp-X, eines cholestase-spezifischen Lipoproteins. Dtsch Med Wochenschr 98:1474–1475, 1973

    PubMed  Google Scholar 

  19. Neubeck W, Seidel D: Direct method for measuring lipoprotein-X in serum. Clin Chem 21(7):853–856, 1975

    PubMed  Google Scholar 

  20. Gidez LI, Miller GJ, Burstein M, Slagle S, Eder HA: Separation and quantitation of subclasses of human plasma high density lipoproteins by a simple precipitation procedure. J Lipid Res 23:1206–1223, 1982

    PubMed  Google Scholar 

  21. Mancini G, Carbonara AO, Heremans JF: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235–254, 1965

    PubMed  Google Scholar 

  22. Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC: Enzymatic determination of total serum cholesterol. Clin Chem 20(4):470–475, 1974

    PubMed  Google Scholar 

  23. Wahlefeld AW: Triglycerides determination after enzymatic hydrolisis.In Methods of Enzymatic Analysis. HU Bergmeyer (ed). New York, Academic Press, 1976, pp 1831–1835

    Google Scholar 

  24. Zilversmit DB, Davis AK: Microdetermination of plasma phospholipids by trichloracetic acid precipitation. J Lab Clin Med 35:155–160, 1950

    PubMed  Google Scholar 

  25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951

    PubMed  Google Scholar 

  26. Catapano AL, Jackson RL, Gilliam EB, Gotto AM, Smith LC: Quantification of apo C-II and apo C-III of human very low density lipoproteins by analytical isoelectric focusing. J Lipid Res 19:1047–1052, 1978

    PubMed  Google Scholar 

  27. Huttunen JK, Ehnholm C, Kekki M, NikkilÄ EA: Postheparin plasma lipoprotein lipase and hepatic lipase in normal subjects and in patients with hypertriglyceridaemia: Correlations to sex, age and various parameters of triglyceride metabolism. Clin Sci Mol Med 50:249–260, 1976

    PubMed  Google Scholar 

  28. Guenther WC: Analysis of Variance. Englewood Cliffs, New Jersey, Prentice-Hall Inc, 1964, p 57

    Google Scholar 

  29. Ritland S, Blomhoff JP, Gjone E: Lecithin:cholesterol acyltransferase and lipoprotein-X in liver disease. Clin Chim Acta 49:251–259, 1973

    PubMed  Google Scholar 

  30. Patsch W, Patsch JR, Kunz F, Sailer S, Braunsteiner H: Studies on the degradation of lipoprotein-X. Eur J Clin Invest 7:523–530, 1977

    PubMed  Google Scholar 

  31. Ritland S, Stokke KT, Gjone E: Changes in the concentration of lipoprotein-X during incubation of postheparin plasma from patients with familial lecithin:cholesterol acyltransferase (LCAT) deficiency. Clin Chim Acta 67:63–69, 1976

    PubMed  Google Scholar 

  32. Rubiés-Prat J, Ras MR, Frisón JC, Miquel C, Masdeu S, Bacardi R: Effect of heparin on lecithin:cholesterol acyltransferase and lipids in cholestasis. Digestion 14:250–255, 1976

    PubMed  Google Scholar 

  33. Sauar J, Holme R, Blomhoff JP: Triglyceride lipase activity in post heparin plasma lipoproteins in liver disease. Clin Chim Acta 87:327–340, 1978

    PubMed  Google Scholar 

  34. Jansen H, Hulsmann WC: Heparin-releasable (liver) lipase(s) may play a role in the uptake of cholesterol by steroid-secreting tissues. Trends Biochem Sci 5:265–268, 1980

    Google Scholar 

  35. Jansen H: Inhibition of liver lipasein vivo leads to induction ofde novo cholesterol synthesis in rat liver. Biochem Biophys Res Commun 131(2):574–578, 1985

    PubMed  Google Scholar 

  36. Kuusi T, Saarinen P, NikkilÄ EA: Evidence for the role of hepatic endothelial lipase in the metabolism of plasma high density lipoprotein 2 in man. Atherosclerosis 36:589–593, 1980

    PubMed  Google Scholar 

  37. Jansen H, Van Tol A, Hülsmann WC: On metabolic function of heparin releasable liver lipase. Biochem Biophys Res Commun 92(1):53–59, 1980

    PubMed  Google Scholar 

  38. Van Tol A, Van Gent T, Jansen H: Degradation of high density lipoprotein by heparin-releasable liver lipase. Biochem Biophys Res Com 94(1): 101–108, 1980

    PubMed  Google Scholar 

  39. Grosser J, Schrecker O, Greten H: Function of hepatic triglyceride lipase in lipoprotein metabolism. J Lipid Res 22:437–442, 1981

    PubMed  Google Scholar 

  40. Kubo M, Matsuzawa Y, Tajima S, Ishikawa K, Yamamoto A, Tarui S: Apo A-I and apo A-II inhibit hepatic triglyceride lipase from human post-heparin plasma. Biochem Biophys Res Commun 100(1):261–266, 1981

    PubMed  Google Scholar 

  41. Jahn CE, Osborne JC, Schaefer EJ, Brewer HB Jr: Activation of the enzymic activity of hepatic lipase by apolipoprotein A-II. Characterization of a major component of high density lipoprotein as activating componentin vitro. Eur J Biochem 131:25–29, 1983

    PubMed  Google Scholar 

  42. Patton JS, Carey MC: Inhibition of human pancreatic lipasecolipase activity by mixed bile salt-phospholipid micelles. Am J Physiol 4(4)G328-G336, 1981

    Google Scholar 

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Authors and Affiliations

  1. Department of Internal Medicine, First Division of Medical Pathology, Policlinico, University of Padua, Padova, Italy

    Goretta Baldo-Enzi BS, Maria Rosa Baiocchi BS, Massimo Grotto MD, Anna Rosa Floreani MD, Manuela Zagolin MD, Maria Chiaramonte MD, Franco Cera BS & Renato Fellin MD

  2. Department of Internal Medicine, Division of Gastroenterology, Policlinico, University of Padua, Padova, Italy

    Goretta Baldo-Enzi BS, Maria Rosa Baiocchi BS, Massimo Grotto MD, Anna Rosa Floreani MD, Manuela Zagolin MD, Maria Chiaramonte MD, Franco Cera BS & Renato Fellin MD

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  1. Goretta Baldo-Enzi BS
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Baldo-Enzi, G., Baiocchi, M.R., Grotto, M. et al. Lipoprotein pattern and plasma lipoprotein lipase activities in patients with primary biliary cirrhosis. Digest Dis Sci 33, 1201–1207 (1988). https://doi.org/10.1007/BF01536666

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  • DOI: https://doi.org/10.1007/BF01536666

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