Abstract
Increases in intracellular calcium ion mediate smooth muscle contraction, but the requirement for extracellular calcium availability during this process is unclear. We studied the intact guinea pig gallbladder in an organ bath to define the effect of low bath calcium on contractile responses to varying doses of cholecystokinin-octapeptide, histamine, and acetylcholine. The contractile responses to cholecystokininoctapeptide and acetylcholine were dependent on the presence of calcium in the bath, with a 40–100% reduction in contraction when buffer with no added calcium was used. In contrast, the contractile response to histamine was unchanged when stimulation was carried out in low calcium buffer. The contractions of the guinea pig gallbladder induced by cholecystokininoctapeptide and acetylcholine, but not histamine, appear to require near-physiologic levels of extracellular calcium.
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References
Hartshorne DJ, Siemankowski RF: Regulation of smooth muscle actomyosin. Annu Rev Physiol 43:519–530, 1981
Ryan JP: Calcium and gallbladder smooth muscle contraction in the guinea pig: Effect of pregnancy. Gastroenterology 89:1279–1285, 1985
Goldenberg MM, Meurer RD: The effect of calcium antagonists on contractions of the sensitized and normal guinea pig ileum. Prostaglandins 26:615–622, 1983
Bostwick J, Baer R, Wang MB, Ryan JP: Effect of verapamil on gallbladder (GB) smooth muscle contractionin vitro. Fed Proc 46:2150a, 1987
Bitar KN, Burgess GM, Putney JW Jr, Makhlouf GM: Source of activator calcium in isolated guinea pig and human gastric muscle cells. Am J Physiol 250:G280-G286, 1986
Hallenbeck GA: Biliary and pancreatic intraductal pressures. In Handbook of Physiology, CF Code (ed). American Physiological Society, Washington DC, 1968, pp. 1007–1025
Brotschi EA, LaMonte WW, Williams LF Jr: Effect of dietary cholesterol and indomethacin on cholelithiasis and gallbladder motility in the guinea pig. Dig Dis Sci 29:1050–1057, 1984
Schoetz DJ, LaMorte WW, Wise WE, Birkett DH, Williams LF Jr: Mechanical properties of primate gallbladder: Description of a dynamic method. Am J Physiol 241:G376-G381, 1981
Grider JR, Maklouf GM: Regional and cellular heterogeneity of cholecystokinin receptors mediating muscle contraction in the gut. Gastroenterology 92:175–180, 1987
Hurwitz L, Weissinger J: Effects of variations in extracellular acetylcholine and calcium ion concentration on the operational level of calcium channels in intestinal smooth muscle. J Pharmacol Exp Ther 214:581–588, 1980
Prosser CL: Smooth muscle. Annu Rev Physiol 38:503–534, 1976
Tomiyama A, Takayanagi I, Takagi K: Relationships between Ca2+ uptake by a microsomal fraction of guinea-pig taenia caecum and its relaxation. Biochem Pharmacol 24:9–12, 1975
Ford GD, Hess ML: Influence of ATP on sarcoplasmic reticulum function of vascular smooth muscle. Am J Physiol 242:C242-C249, 1982
Shibata N, Ohashi H, Takewaki T, Okada T: Calcium source for contractile response of guinea pig taenia caecum to carbachol in a calcium deficient, potassium rich solution. Jpn J Pharmacol 28:561–568, 1978
Ohashi H, Takewaki T, Okada T: Calcium and the contractile effect of carbachol in the depolarized guinea pig taenia caecum. Jpn J Pharmacol 24:601–611, 1974
Somlyo AV, Vinall P, Somlyo AP: Excitation contraction coupling and electrical events in two types of vascular smooth muscle. Microvasc Res 1:354–373, 1969
Goldenberg MM, Meurer RD: The effect of calcium antagonists on contractions of the sensitized and normal guinea pig ileum. Prostaglandins 26:615–622, 1983
Kovaki H, Weiss GB: Calcium channels in smooth muscle. Gastroenterology 87:960–970, 1984
Cauvin C, Lontzenhiser R, Van Breemen C: Mechanisms of calcium antagonist-induced vasodilation. Annu Rev Pharmacol Toxicol 23:373–396, 1983
Mayer CJ, Van Breemen C, Casteels R: The actions of lanthanum and D600 on the calcium exchange in the smooth muscle cells of the guinea pig taenia coli. Pflugers Arch 337:333–350, 1972
Deth R, Van Breeman C: Relative contributions of Ca2+ influx and cellular Ca2+ release during drug induced activation of rabbit aorta. Pflugers Arch 348:13–22, 1974
Yamamura T, Takahashi T, Kusunoki M, Kantoh M, Ishikawa Y, Utsunomiya J: Cholecystokinin-octapeptide evoked3H-acetylcholine release from guinea pig gallbladder. Neurosci Lett 65:167–170, 1986
Takahashi T, Yamamura T, Ishikawa Y, Kantoh M, Utsunomiya J: Effects of cholecystokinin-octapeptide on the human gallbladder bothin vivo andin vitro. Gastroenterol Jpn 21:49–54, 1986
Rubin RP: The role of calcium in the release of neurotransmitter substances and hormones. Pharmacol Rev 22:389–428, 1970
Bitar KN, Mikhlouf GM: Receptors on smooth muscle cells—characterization by contraction and specific antagonists. Am J Physiol 242:G400-G407, 1982
Casteels R, Raeymaekers L: The action of acetylcholine and catecholamines on an intracellular calcium store in the smooth muscle cells of the guinea pig taenia coli. J Physiol 294:51–68, 1979
La Morte WW, Hingston SJ, Wise WE: pH-dependent activity of H1 and H2-histamine receptors in guinea pig gallbladder. J Pharmacol Exp Ther 217:638–644, 1981
Schoetz DJ, Birkett DH, Williams LF Jr: Gallbladder motor function in the intact primate: Autonomic pharmacology. J Surg Res 24:513–519, 1978
Mangel AW, Nelson DO, Rabovsky JL, Presser CL, Connor JA: Depolarization-induced contractile activity of smooth muscle in calcium-free solution. Am J Physiol 242:C36-C40, 1982
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This research was supported by NIH grant S07-RR05487-22.
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Brotschi, E.A., Crocker, K.C., Gianitsos, A.N. et al. Effect of low extracellular calcium on gallbladder contractionin vitro . Digest Dis Sci 34, 360–366 (1989). https://doi.org/10.1007/BF01536256
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DOI: https://doi.org/10.1007/BF01536256