Abstract
We have created somatic cell hybrids between the temperature-sensitive mouse cell line tsA1S9 and human cell lines in order to localize the human gene (A1S9T)complementing the cell cycle defect of the murine line. Segregation of the human X chromosome is completely concordant with growth at the nonpermissive temperature. Hybrids retaining the X chromosome are temperature-resistant, whereas those without a human X are temperature-sensitive. Further hybrids made using human cell lines with X-autosome translocations indicate that the A1S9T gene is located on the short arm of the human X chromosome.
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Brown, C.J., Powers, V.E., Munroe, D.L. et al. Gene on short arm of human X chromosome complements murine tsA1S9 DNA synthesis mutation. Somat Cell Mol Genet 15, 173–178 (1989). https://doi.org/10.1007/BF01535079
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DOI: https://doi.org/10.1007/BF01535079