Abstract
Vasoactive intestinal peptide (VIP) is a major neuropeptide involved in multiple functions such as vasodilation, smooth-muscle relaxation, sweat secretion, gastrointestinal peristalsis, pancreatic function, and brain activity. In view of the multiple roles associated with VIP,it is important to understand its gene regulation. We have recently isolated the human VIPgene and determined its structure. By in situ hybridization techniques we have now localized this gene to the long arm of chromosome 6, 6q24, a chromosomal region that has been shown previously to contain the coding sequences for the nuclear protooncogene c-myb.Genes found in close proximity to each other on the chromosome are often functionally related and, as VIPis primarily expressed in the nervous system, we investigated the possible correlation of c-mybto VIPin neuronal tissue. A sharp peak of c-mybmRNA was observed in the hippocampus of 3-day-old rats, preceding the peak of VIPmRNA that occurs in this brain area at 8 days of age. Thus, the protooncogene c-mybmay be associated with events in brain development occurring prior to the appearance of elevated concentrations of VIP.
Similar content being viewed by others
Literature cited
Said, S.I. (1986).J. Endocrinol. Invest. 9:191–200.
Brenneman, D.E., and Eiden, L.E. (1986).Proc. Natl. Acad. Sci. U.S.A. 83:1159–1162.
Stanisz, A.M., Befus, D., and Bienenstock, J. (1986).J. Immunol. 136:152–156.
Zabel, B.U., Naylor, S.L., Sakaguchi, A.Y., Bell, G.I., and Shows, T.B. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:6932–6936.
Bodner, M., Fridkin, M., and Gozes, I. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:3548–3551.
Gozes, I., Bodner, M., Shani, Y., and Fridkin, M. (1986).Peptides 7:1–6.
Pelicci, P.-G., Lanfrancone, L., Brathwaite, M.D., Wolman, S.R., and Dalla-Favera, R. (1984).Science 224:1117–1121.
Harper, M.E., Franchini, G., Love, J., Simon, M.I., Gallo, R.C., and Wong-Staal, F. (1983).Nature 304:169–171.
Bishop, M.J. (1985).TIG 1:245–249.
Ralston, R., and Bishop, M.J. (1983).Nature 306:803–806.
Westin, E.H., Gallo, R.C., Arya, S.K., Sonza, L.M., Baluda, M.A., Aaronson, S.A., and Wong-Staal, F. (1982).Proc. Natl. Acad. Sci. U.S.A. 79:2194–2198.
Gozes, I., Shani, Y., and Rostene, W.H. (1987).Mol. Brain Res. (in press).
Tsukada, T., Horovitz, S.J., Montminy, M.R., Mandal, G., and Goodman, R.H. (1985).DNA 4:293–300.
Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K., and Green, M.R. (1984).Nucleic Acid Res. 12:7035–7056.
Cox, K.H., Deleon, D.V., Angerer, L.M., and Angerer, R.C. (1984).Dev. Biol. 101:485–502.
Itoh, N., Obata, K.I., Yanaihara, N., and Okamoto, H. (1983).Nature 304:547–549.
Verma, I.M. (1977).Biochim. Biophys. Acta 473:1–38.
Maxam, A., and Gilbert, W. (1980).Methods Enzymol. 65:499–560.
Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982).Molecular Cloning: A Laboratory Manual, (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York).
Aviv, H., and Leder, P. (1972).Proc. Natl. Acad. Sci. U.S.A. 69:1408–1412.
Gozes, I., Bodner, M., Shwartz, H., Shani, Y., and Fridkin, M. (1984).Peptides 5:161–166.
Rigby, P.W.J., Dieckman, M., Rhodes, C., and Berg, P. (1977).J. Mol. Biol. 113:237–251.
Gozes, I., Avidor, R., Katznelson, D., Yahav, Y., Croce, C., and Huebner, K. (1987).Hum. Genet. 75:41–44.
Nobou, F., Besson, J., Rostene, W., and Rosselin, G. (1985).Dev. Brain Res. 20:296–301.
Gozes, I. (1987). InBrain Peptides Update, (eds.) Martin, J.B., Brownstein, M.J., and Krieger, D. (John Wiley & Sons, New York) pp. 141–162.
Weinstein, Y., Ihle, J.N., Lavu, S., and Prekumer Reddy, E. (1986).Proc. Natl. Acad. Sci. U.S.A. 83:5010–5014.
Gonda, T.J., Gough, N.M., Dunn, A.R., and de Blaquiere, J. (1985).EMBO J. 8:2003–2008.
Ginzburg, I., Teichman, A., Dodemont, H.J., Behar, L., and Littauer, U.Z. (1985).EMBO J. 4:3667–3673.
Wolf, D., Laver-Rudich, Z., and Rotter, V. (1985).Mol. Cell. Biol. 5:1887–1893.
Wolf, D., and Rotter, V. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:790–794.
Ginzburg, I., and Littauer, U.Z. (1982). InMolecular Biology of the Cytoskeleton, (eds.), Borisy, G.G., Cleveland, D.W., and Murphy, D.B. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York) pp. 357–366.
Bell, G.I., Gerhard, D.S., Fong, N.M., Sanchez-Pescador, R., and Rall, L.B. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:6450–6454.
Marie, J.C., Hui Bon Hoa, D., Hejblum, G., and Rosselin, G. (1985).Regul. Peptides, Suppl. 3:S33.
Norton, W.T.B., and Poduslo, S.E. (1973).J. Neurochem. 21:759–773.
Berelowitz, M., Hudson, A., Pimstone, A., and Kronheim, S. (1978).J. Neurochem. 31:751–753.
Duprey, S.P., and Boettiger, D. (1985).Proc. Natl. Acad. Sci. U.S.A. 82:6937–6941.
Thompson, C.B., Challoner, P.B., Neiman, P.E., and Groudine, M. (1986).Nature 319:374–380.
Rappoport, D.A., and Fritz, R.R. (1972) InThe Structure and Function of the Nervous Tissue, Vol. VI, (ed.) Bourne, G.H. (Academic Press, New York) pp. 273–316.
Schultze, B., Nowak, B., and Maurer, W. (1974).J. Comp. Neurol. 158:207–218.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gozes, I., Nakai, H., Byers, M. et al. Sequential expression in the nervous system of C-MYB andVIP genes, located in human chromosomal region 6q24. Somat Cell Mol Genet 13, 305–313 (1987). https://doi.org/10.1007/BF01534924
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01534924