Skip to main content
SpringerLink
Log in

Selection of highly transfectable variant from mouse mastocytoma P815

  • Published:
Somatic Cell and Molecular Genetics

Abstract

A tk− cell line derived from mouse mastocytoma P815 was transfected with a plasmid carrying a thymidine kinase gene. The tk+ cells were obtained at a frequency of 10−6. From some of these tk+ transfectants it was possible to select tk− cells with BrdU so that these cells could be submitted again to transfection with the thymidine kinase gene. By repeating cycles of transfection and tk+ selection followed by reverse selection with BrdU, it was possible to obtain a stable variant having a 100-fold increase in transfection efficiency. This HTR (high transfection) variant shows a high efficiency of transfection (10−4) for the neomycin-resistance gene as well as for the thymidine kinase gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  1. Lowy, I., Pellicer, A., Jackson, J.F., Sim, G.K., Silverstein, S., and Axel, R. (1980).Cell 22:817–823.

    PubMed  Google Scholar 

  2. Perucho, M., Hanahan, D., Lipsich, L., and Wigler, M. (1980).Nature 285:207–210.

    PubMed  Google Scholar 

  3. Bradshaw, H.D. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:5588–5591.

    PubMed  Google Scholar 

  4. Margulies, D.H., Evans, G.A., Ozato, K., Camerini-Otero, R.D., Tanaka, K., Appella, E., and Seidman, J.G. (1983).J. Immunol. 130:463–470.

    PubMed  Google Scholar 

  5. Kuhn, L.C., McClelland, A., and Ruddle, F.H. (1984).Cell 37:95–103.

    PubMed  Google Scholar 

  6. Shih, C., Padhy, L.C., Murray, M., and Weinberg, R.A. (1981).Nature 290:261–264.

    PubMed  Google Scholar 

  7. Goodenow, R.S., Stroynowski, I., McMillan, M., Nicolson, M., Eakle, K., Taylor Sher, B., Davidson, N., and Hood, L. (1983).Nature 301:388–394.

    PubMed  Google Scholar 

  8. Graham, F.L., and van der Eb, A.J. (1973).Virology 52:456–467.

    PubMed  Google Scholar 

  9. Weinberg, R.A. (1981).Biochim. Biophys. Acta 651:25–35.

    PubMed  Google Scholar 

  10. Southern, P.J., and Berg, P. (1982).J. Mol. Appl. Genet. 1:327–341.

    PubMed  Google Scholar 

  11. Wigler, M., Sweet, R., Sim, G.K., Wold, B., Pellicer, A., Lacy, E., Maniatis, T., Silverstein, S., and Axel, R. (1979).Cell 16:777–785.

    PubMed  Google Scholar 

  12. Kavathas, P., and Herzenberg, L.A. (1983).Proc. Natl. Acad. Sci. U.S.A. 80:524–528.

    PubMed  Google Scholar 

  13. Kuhn, L.C., Barbosa, J.A., Kamarck, M.E., and Ruddle, F.H. (1983).Mol. Biol. Med. 1:335–352.

    PubMed  Google Scholar 

  14. Neuberger, M.S. (1983).EMBO J. 2:1373–1378.

    PubMed  Google Scholar 

  15. Gillies, S., Morrison, S., Oi, V., and Tonegawa, S. (1983).Cell 33:717–728.

    PubMed  Google Scholar 

  16. Ott, M.-O., Sperling, L., Herbomel, P., Yaniv, M., and Weiss, M.C. (1984).EMBO J. 3:2505–2510.

    PubMed  Google Scholar 

  17. Uyttenhove, C., Van Snick, J., and Boon, T. (1980).J. Exp. Med. 152:1175–1183.

    PubMed  Google Scholar 

  18. Maryanski, J.L., Szpirer, J., Szpirer, C., and Boon, T. (1983).Somat. Cell Genet. 9:345–357.

    PubMed  Google Scholar 

  19. Corsaro, C., and Pearson, M.L. (1981).Somat. Cell Genet. 7:603–616.

    PubMed  Google Scholar 

  20. Ozato, K., Hansen, T.H., and Sachs, D.H. (1980).J. Immunol. 125:2473–2477.

    PubMed  Google Scholar 

  21. Tonkonogy, S.L., and Amos, D.B. (1981).Immuno-genetics 14:497–505.

    Google Scholar 

  22. Maryanski, J.L., Van Snick, J., Cerottini, J.-C., and Boon, T. (1982).Eur. J. Immunol. 12:401–406.

    PubMed  Google Scholar 

  23. Uyttenhove, C., Maryanski, J.L., and Boon, T. (1983).J. Exp. Med. 157:1040–1052.

    PubMed  Google Scholar 

  24. Colbere-Garapin, F., Chousterman, S., Horodniceanu, F., Kourilsky, P., and Garapin, A.C. (1979).Proc. Natl. Acad. Sci. U.S.A. 76:3755–3759.

    PubMed  Google Scholar 

  25. Nicolas, J.F., and Berg, P. (1983). Teratocarcinoma stem cells. Cold Spring Harbor Conferences on Cell Proliferation, Vol. 10, pp. 469–485.

    Google Scholar 

  26. Davis, R.W., Botstein, D., and Roth, J.R. (1980). InA Manual for Genetic Engineering. (Cold Spring Harbor Laboratory, Cold Spring Harbor, New York), pp. 116–119.

    Google Scholar 

  27. Gross-Bellard, M., Oudet, P., and Chambon, P. (1973).Eur. J. Biochem. 36:32–38.

    PubMed  Google Scholar 

  28. Heidmann, O., and Rougeon, F. (1982).Cell 28:507–513.

    PubMed  Google Scholar 

  29. Wigler, M., Pellicer, A., Silverstein, S., and Axel, R. (1978).Cell 14:725–731.

    PubMed  Google Scholar 

  30. Perucho, M., Hanahan, D., and Wigler, M. (1980).Cell 22:309–317.

    PubMed  Google Scholar 

  31. Pellicer, A., Robins, D., Wold, B., Sweet, R., Jackson, J., Lowy, I., Roberts, J.M., Sim, G.K., Silverstein, S., and Axel, R. (1980).Science 209:1414–1422.

    PubMed  Google Scholar 

  32. Clough, D.W., Kunkel, L.M., and Davidson, R.L. (1982).Science 216:70–73.

    PubMed  Google Scholar 

  33. Ostrander, M., Vogel, S., and Silverstein, S. (1982).Mol. Cell. Biol. 2:708–714.

    PubMed  Google Scholar 

  34. Christy, B.A., and Scangos, G.A. (1984).Mol. Cell. Biol. 4:611–617.

    PubMed  Google Scholar 

  35. Davies, R.L., Fuhrer-Krusi, S., and Kucherlapati, R.S. (1982).Cell 31:521–529.

    PubMed  Google Scholar 

  36. Kaufman, E.R., and Davidson, R.L. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:4982–4986.

    PubMed  Google Scholar 

  37. Boon, T. (1983).Adv. Cancer Res. 39:121–151.

    PubMed  Google Scholar 

  38. Bernarbeu, C., Finlay, D., van de Rijn, M., Maziarz, R., Biro, A., Spits, H., de Vries, J. and Terhorst, C. (1983).J. Immunol. 131:2032–2037.

    PubMed  Google Scholar 

  39. Maryanski, Y.L., Moretta, A., Jordan, B., De Plaen, E., Van Pel, A., Boon, T. and Cerottini, Y-C. (1985)Eur. Y. Immunol., in press.

Download references

Author information

Authors and Affiliations

  1. Ludwig Institute for Cancer Research, 74 avenue Hippocrate, UCL 74.59-1200, Brussels, Belgium

    Aline Van Pel, Etienne De Plaen & Thierry Boon

  2. Cellular Genetics Unit, Catholic University of Louvain, Belgium

    Aline Van Pel, Etienne De Plaen & Thierry Boon

Authors
  1. Aline Van Pel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Etienne De Plaen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Thierry Boon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Van Pel, A., De Plaen, E. & Boon, T. Selection of highly transfectable variant from mouse mastocytoma P815. Somat Cell Mol Genet 11, 467–475 (1985). https://doi.org/10.1007/BF01534840

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01534840

Keywords

Search

Navigation