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Differences in altered expression of L-selectin and Mac-1 in monocytes and neutrophils

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Abstract

We have measured the altered expression of the adhesion-promoting glucoproteins L-selectin and Mac-1 on monocytes and neutrophils after incubation with different concentrations of the chemotactic factor FMLP. We found a time-and doserelated down-regulation of L-selectin on both monocytes and neutrophils, which was more pronounced on neutrophils as compared to monocytes at both low (10−12 M) and high (10−7 M) concentrations of the chemotactic factor FMLP. Presence of human albumin reduced the down-regulation of L-selectin on both monocytes and neutrophils but to a greater extent on monocytes. Mac-1 expression increased in a time and dose-dependent manner on both monocytes and neutrophils when they were incubated with both low (10−12 M) and high (10−7 M) concentrations of FMLP, but the expression was more pronounced on neutrophils. The up-regulation of Mac-1 was equally reduced by albumin on monocytes and neutrophils. Presence of EDTA in RPMI medium reduced the FMLP-induced down-regulation of L-selectin on both monocytes ad neutrophils, indicating that the down-regulation of L-selectin is partly divalent cation dependent. Differences in the altered expression of L-selectin and Mac-1 in monocytes and neutrophils with sustained expression of L-selectin on monocytes can contribute to the sequential recruitment of these cells into an inflammatory site.

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  1. Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden

    J. Lundahl & J. Hed

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  1. J. Lundahl
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  2. J. Hed
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Lundahl, J., Hed, J. Differences in altered expression of L-selectin and Mac-1 in monocytes and neutrophils. Inflammation 18, 67–76 (1994). https://doi.org/10.1007/BF01534599

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