Skip to main content
SpringerLink
Log in

Endothelin-1 does not prime polymorphonuclear leukocytes for enhanced production of reactive oxygen metabolites

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

The in vitro effect of endothelin-1 (ET-1) on the capacity of polymorphonuclear leukocytes (PMNLs) to generate reactive oxygen species (ROS) was investigated. Human PMNLs were separated from healthy volunteers and preincubated for 10 min. at 37°C with varying concentrations (10−7–10−12 M) of ET-1. After subsequent stimulation with FMLP (10−7 M) or opsonized zymosan (0.5 mg/ ml) the intra- and extracellular generation of ROS was assessed by luminol-amplified chemiluminescence, superoxide radical (·O −2 ) and hydrogen peroxide (H2O2) production.Results: ET-1 alone failed to stimulate ROS generation. Neither the capacity for extracellular generation of oxygen metabolites nor the production of ROS with an intracellular origin was changed after preincubation of PMNLs with ET-1. ET-1 did not cause a shift of the ·O −2 /H2O2 production ratio after stimulation of PMNLs with FMLP. These findings suggest that ET-1 in vitro does not prime human PMNLs for enhanced production of ROS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Battistini, B., P. D'orleans-Juste, andP. Sirois. 1993. Biology of disease. Endothelins: Circulating plasma levels and presence in other biological fluids.Lab. Invent. 68:600–628.

    Google Scholar 

  2. Takuwa Y. Endothelin in vascular and endocrcine systems: biological activities and its mechanisms of action. 1993.Endocrine J. 40:489–506.

    Google Scholar 

  3. Naruse, M., M. Kawana, S. Hifumi, K. Naruse, I. Yoshihara, T. Oka, et al. 1991. Plasma immunoreactive endothelin, but not thrombomodulin, is increased in patients with essential hypertension and ischemic heart disease. 1991.J. Cardiovasc. Pharmacol. 17(Suppl. 7):S471-S474.

    PubMed  Google Scholar 

  4. Krämer, B. K., M. Ackermann, S. M. Kohler, andG. A. J. Riegger. 1994. Role of endothelin in hypertension.Clin. Investig. 72:88–93.

    PubMed  Google Scholar 

  5. Lemne, C. E., T. Lundeberg, E. Theodorsson, andU. De Faire. 1994. Increased basal concentration of plasma endothelin in borderline hypertension.J. Hypertension 12:1069–1074.

    Google Scholar 

  6. Haak, T., W. März, E. Jungmann, S. Hausser, R. Siekmeier, W. Gross, andK.-H. UsaDel. 1994. Elevated endothelin levels in patients with hyperlipoproteinemia.Clin. Investig. 72:580–584.

    PubMed  Google Scholar 

  7. Lerman, A., B. S. Edwards, J. W. Hallet, D. M. Heublein, S. M. Sandberg, andJ. C. Burnett. 1991. Circulating and tissue endothelin immunoreactivity in advanced atherosclerosis.N. Engl. J. Med. 325:997–1001.

    PubMed  Google Scholar 

  8. Wright, C. D., G. P. Hingorani, andS. T. Rapundalo. 1991. Characterization of the human neutrophil receptor for endothelin-1.J. Leukocyte Biol. Suppl.2:92.

    Google Scholar 

  9. Lopez Farre, A., A. Riesco, G. Espinosa, E. Digiuni, M. R. Cernadas, V. Alvarez, M. Monton et al. 1993. Effect of endothelin-1 on neutrophil adhesion to endothelial cells and perfused heart.Circulation 88:1166–1171.

    PubMed  Google Scholar 

  10. Elferink, J. G., andB. M. De Koster. 1994. Endothelin-induced activation of neutrophil migration.Biochem. Pharmacol. 48:865–871.

    PubMed  Google Scholar 

  11. Gomez-Garre, D., M. Guerra, E. Gonzalez, A. Lopez-Farre, A. Riesco, C. Caramelo, J. Escanero, andJ. Egido. 1992. Aggregation of human polymorphonuclear leukocytes by endothelin: role of platelet-activating factor.Eur. J. Pharmacol. 224:167–172.

    PubMed  Google Scholar 

  12. Lopez Farre, A., A. Riesco, M. Moliz, J. Egido, S. Casado, L. Hernando, andC. Car-Amelo. 1991. Inhibition by L-arginine of the endothelin-mediated increase in cytosolic calcium in human neutrophils.Biochem. Biophys. Res. Commun. 178:884–891.

    PubMed  Google Scholar 

  13. Wright, C. D., W. L. Cody, J. B. Dunbar, A. M. Doherty, G. P. Hingorani, andS. T. Rapundalo. 1994. Characterization of endothelins as chemoattractants for human neutrophils.Life Sci. 55:1633–1641.

    PubMed  Google Scholar 

  14. Ishida, K., K. Takeshige, andS. Minakami. 1990. Endothelin-1 enhances superoxide generation of human neutrophils stimulated by the chemotactic peptideN-formyl-methionyl-leucylphenylalanine.Biochem. Biophys. Res. Commun. 173:496–500.

    PubMed  Google Scholar 

  15. Hafström, I., B. Ringertz, T. Lundeberg, andJ. Palmblad. 1993. The effect of endothelin, neuropeptide Y, calcitonin gene-related peptide and substance P on neutrophil functions.Acta Physiol. Scand. 148:341–346.

    PubMed  Google Scholar 

  16. Prasad, K., P. Lee, andJ. Kalra. 1991. Influence of endothelin on cardiovascular function, oxygen free radicals, and blood chemistry.Am. Heart J. 121:178–187.

    PubMed  Google Scholar 

  17. Cohen, H. J., andM. E. Chovaniec. 1978. Superoxide generation by digitonin-stimulated guinea pig granulocytes.J. Clin. Invest. 61:1081–1087.

    PubMed  Google Scholar 

  18. Hyslop, P. A., andL. A. Sklar. 1984. A quantitative fluorimetric assay for the determination of oxidant production by polymorphonuclear leukocytes.Anal. Biochem. 141:280–286.

    PubMed  Google Scholar 

  19. Follin, P., andC. Dahlgren. 1990. Altered O −2 /H2O2 production ratio byin vitro andin vivo primed human neutrophils.Biochem. Biophys. Res. Commun. 167:970–976.

    PubMed  Google Scholar 

  20. Metcalf, J. A., J. I. Gallin, W. M. Nauseef, andR. K. Root. 1986. Laboratory manual of neutrophil function. Raven Press, New York.

    Google Scholar 

  21. Bender, J. G., andD. E. Van Epps. 1983. Analysis of the bimodal chemiluminescence pattern stimulated in human neutrophils by chemotactic factors.Infect. Immun. 41:1062–1070.

    PubMed  Google Scholar 

  22. Dahlgren, C. 1988. Effects on extra- and intracellularly localized, chemoattractant-induced, oxygen radical production in neutrophils following modulation of conditions for ligand-receptor interaction.Inflammation 12:335–349.

    PubMed  Google Scholar 

  23. Briheim, G., P. Follin, S. Sandstedt, andC. Dahlgren. 1989. Relationship between intracellularly and extracellularly generated oxygen metabolites from primed polymorphonuclear leukocytes ditfers from that obtained from nonprimed cells.Inflammation. 13:455–464.

    PubMed  Google Scholar 

  24. Dahlgren, C., andT. Sundquist. 1991. The effects of N-ethylmaleimide on extracellularly and intracellularly generated chemiluminescence in neutrophils indicate that the rate of deactivation of NADPH oxidase is higher when the oxidase system is localized on the plasma membrane than when it is localized on the phagosomal membrane.J. Biolum. Chemilum. 6:81–86.

    Google Scholar 

  25. Halstensen, A., B. Haneberg, J. Glette, S. Sandberg, andC. O. Solberg. 1986. Factors important for the measurement of chemiluminescence production by polymorphonuclear leukocytes.J. Immunol. Meth. 88:121–128.

    Google Scholar 

  26. Ginsburg, I., R. Misgav, D. F. Gibbs, J. Varani, andR. Kohen, 1993. Chemiluminescence in activated human neutrophils: Role of buffers and scavengers.Inflammation 17:227–243.

    PubMed  Google Scholar 

  27. Tennenberg, S. D., andJ. S. Solomkin. 1990. Activation of neutrophils by cachectin/tumor necrosis factor: Priming of N-formyl-methionyl-leucyl-phenylalanine-induced oxidative responsiveness via receptor mobilization without degranulation.J. Leukocyte Biol. 47:217–223.

    PubMed  Google Scholar 

  28. Seifert, R., G. Hilgenstock, M. Fassbender, andA. Distler. 1991. Regulation of the superoxide-forming NADPH oxidase is not altered in essential hypertension.J. Hypertension 9:147–153.

    Google Scholar 

  29. Follin, P., A. Johansson, andC. Dahlgren. 1991. Intracellular production of reactive oxygen species in human neutrophils following activation by the soluble stimuli FMLP, dioctanoylglycerol and ionomycin.Cell Biochem. Funct. 3:29–37.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Carl Gustav Carus Medical School Department of Internal Medicine III Pathological Biochemistry, Technical University of Dresden, Fetscherstr. 74, D-01307, Dresden, Germany

    Steffi Kopprasch, A. Gatzweiler, M. Kohl & H. -E. Schröder

Authors
  1. Steffi Kopprasch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Gatzweiler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Kohl
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. -E. Schröder
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kopprasch, S., Gatzweiler, A., Kohl, M. et al. Endothelin-1 does not prime polymorphonuclear leukocytes for enhanced production of reactive oxygen metabolites. Inflammation 19, 679–687 (1995). https://doi.org/10.1007/BF01534571

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01534571

Keywords

Search

Navigation