Skip to main content
SpringerLink
Log in

Recruitment of helper T cells for induction of tumour rejection by cytolytic T lymphocytes

  • Short Communication
  • Tumour Rejection, CTL, Helper Cells
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Abstract

Immunotherapy of cancer could be possible in cases in which competent effector T cells can be induced. Such an approach depends on expression of tumour-specific antigens by the tumour cells and on the availability of sufficient costimulatory support for activation of cytotoxic T lymphocytes. Here, a strategy for helper T cell recruitment for induction of tumour-specific cytotoxic immune responses is presented. Allogenic MHC class II molecules were introduced into tumour cells by cell fusion. These hybrid cells, when injected into mice, induced rejection of an established tumour. The contribution of CD4-expressing helper T cells in the induction phase and of CD8-expressing T cells in the effector phase of the immune response was demonstrated. The approach described could be applicable to cases in which a suitable tumour antigen is present but not identified; it employs regulatory interactions that govern physiological immune responses and is designed to be minimally invasive.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Burnet FM (1971) Immunological surveillance in neoplasia. Transplant Rev 7: 3

    PubMed  Google Scholar 

  2. Boon T (1992) Toward a genetic analysis of tumor rejection antigens. Adv Cancer Res 58: 177

    PubMed  Google Scholar 

  3. Franksson L, Petersson M, Kiessling R, Kärre K (1993) Immunization against tumor and minor histocompatibility antigens by eluted cellular peptides loaded on antigen processing defective cells. Eur J Immunol 23: 2606

    PubMed  Google Scholar 

  4. Melief CJM (1992) Tumor eradication by adoptive transfer of cytotoxic T lymphocytes. Adv Cancer Res 58: 143

    PubMed  Google Scholar 

  5. Schendel DJ, Gansbacher B, Oberneder R, Kriegmair M, Hofstetter A, Riethmüller G, Segurado OG (1993) Tumor-specific lysis of human renal cell carcinomas by tumor-infiltrating lymphocytes. J Immunol 151: 4209

    PubMed  Google Scholar 

  6. Yasumura S, Hirabayashi H, Schwartz DR, Toso JF, Johnson JT, Herberman RB, Whiteside TL (1993) Human cytotoxic T-cell lines with restricted specificity for Squamous-cell carcinoma of the head and neck. Cancer Res 53: 1461

    PubMed  Google Scholar 

  7. Ioannides CG, Whiteside TL (1993) T-cell recognition of human tumors — implications for molecular immunotherapy of cancer. Clin Immunol Immunpathol 66: 91

    Google Scholar 

  8. Maas RA, Dullens HFJ, DenOtter W (1993) Interleukin-2 in cancer treatment — disappointing or (still) promising. Cancer Immunol Immunother 36: 141

    PubMed  Google Scholar 

  9. Colombo MP, Mattei S, Parmiani G (1992) Cytokine gene transfer in tumor cells as an approach to antitumor therapy. Int J Lab Res 21: 278

    Google Scholar 

  10. Hock H, Dorsch M, Kunzendorf U, Qin ZH, Diamantenstein T, Blankenstein T (1993) Mechanisms of rejection induced by tumor cell-targeted gene transfer of interleukin-2, interleukin-4, interleukin-7, tumor necrosis factor or interferon-γ. Proc. Natl Acad Sci USA 90: 2774

    PubMed  Google Scholar 

  11. Chen LP, Ashe S, Brady WA, Hellstrom I, Hellstrom KE, Ledbetter JA, Mcgowan P, Linsley PS (1992) Costimulation of antitumor immunity by the B7 counterreceptor for the lymphocyte-T molecule CD28 and CTLA4. Cell 71: 1093

    PubMed  Google Scholar 

  12. Townsend SE, Allison JP (1993) Tumor rejection after direct costimulation of CD8+ T cells by B7-transfected melanoma cells. Science 259: 368

    PubMed  Google Scholar 

  13. Stuhler G, Walden P (1993) Collaboration of helper and cytotoxic T lymphocytes. Eur J Immunol 23: 2279

    PubMed  Google Scholar 

  14. Gorer PA (1950) Studies in antibody response of mice to tumour inoculation. Br J Cancer 4: 372

    PubMed  Google Scholar 

  15. Kärre K, Ljunggren HG, Piontek G, Kiessling R (1986) Selective rejection of H-2-deficient lymphoma variants suggests alternative immune defence strategy. Nature 319: 675

    PubMed  Google Scholar 

  16. Kim KJ, Kanellopoulos-Langevin C, Merwin RM, Sachs DH, Asofsky R (1979) Establishment and characterization of BALB/c lymphoma lines with B cell properties. J Immunol 122: 549

    PubMed  Google Scholar 

  17. Chang DC, Chassy BM, Saunders JA, Sower AE (eds) (1992) Guide to electroporation and electrofusion. Academic Press, San Diego

    Google Scholar 

  18. Fischer AG, Goff LK, Lightstone L, Marvel J, Mitchison NA, Poirier G, Stauss H, Zamoyska R (1989) Problems in the physiology of class I and class II MHC molecules, and of CD45. CSH Symp Quant Biol 54: 667

    Google Scholar 

  19. Guo Y, Wu M, Chen H, Wang X, Liu G, Lu G, Ma J, Sy M-S (1994) Effective tumor vaccine generated by fusion of hepatoma cells with activated B cells. Science 263: 518

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Immungenetik, Max-Planck-Institut für Biologie, Corrensstr. 42, D-72076, Tübingen, Germany

    Gernot Stuhler & Peter Walden

Authors
  1. Gernot Stuhler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Walden
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stuhler, G., Walden, P. Recruitment of helper T cells for induction of tumour rejection by cytolytic T lymphocytes. Cancer Immunol Immunother 39, 342–345 (1994). https://doi.org/10.1007/BF01519989

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01519989

Key words

Search

Navigation