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A protein fraction from aged garlic extract enhances cytotoxicity and proliferation of human lymphocytes mediated by interleukin-2 and concanavalin A

  • Original Articles
  • Fraction 4, Aged Garlic Extract, Peripheral Blood Lymphocytes, Interleukin-2, Concanavalin A
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Abstract

Fraction 4 (F4), a protein fraction isolated from aged garlic extract, enhanced cytotoxicity of human peripheral blood lymphocytes (PBL) against both naturalkiller (NK)-sensitive K562 and NK-resistant M14 cell lines. Although F4 treatment alone increased cytotoxicity, the effect was more remarkable when F4 was administered together with suboptimal doses of interleukin-2 (IL-2); combination treatment of 5 μg/ml F4 plus 10 U/ml IL-2 for 72 h generated lymphokine-activated killer activity equivalent to that produced by 100 U/ml IL-2 alone against M14. F4 enhanced IL-2-induced proliferation and IL-2 receptor (Tac) expression of PBL without significant increase of IL-2 production. The enhancement of cytotoxicity both by F4 alone and by F4 plus IL-2 was abolished by anti-IL-2 antibody. F4 also enhanced concanavalin-A(ConA)-induced proliferation of PBL. Radiolabeled-ConA binding assays revealed that F4 treatment greatly augmented the affinity and slightly increased the number of ConA binding sites in PBL. F4 also enhanced ConA-induced IL-2 receptor (Tac) expression and IL-2 production of PBL. Anti-IL-2 antibody inhibited the effect of F4 on ConA-induced proliferation. These data suggest that IL-2 is involved in augmentative effects of F4. Our results indicate that F4 is a very efficient immunopotentiator and may be used for immunotherapy.

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Authors and Affiliations

  1. Division of Surgical Oncology, UCLA School of Medicine, 90024, Los Angeles, CA, USA

    Naoko Morioka, Lan L. Sze & Reiko F. Irie

  2. John Wayne Cancer Institute, 2200 Santa, 90404, CZ, Santa Monica, USA

    Donald L. Morton

Authors
  1. Naoko Morioka
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  2. Lan L. Sze
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  3. Donald L. Morton
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  4. Reiko F. Irie
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Morioka, N., Sze, L.L., Morton, D.L. et al. A protein fraction from aged garlic extract enhances cytotoxicity and proliferation of human lymphocytes mediated by interleukin-2 and concanavalin A. Cancer Immunol Immunother 37, 316–322 (1993). https://doi.org/10.1007/BF01518454

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  • DOI: https://doi.org/10.1007/BF01518454

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