Abstract
Spinach chloroplasts pre-incubated at various temperatures, changed their photosynthetic activities (measured at 15°C) as the incubation temperature was raised; these activities showed characteristic activitytemperature profiles as follows:
-
1.
The profiles were shifted to lower temperatures if various aliphatic compounds were present in the incubation mixture.
-
2.
In general, the extent of the shift was proportional to the product of the concentration and the partition coefficient of the compound, i.e. to the concentration of the compound partitioned in the lipophilic chloroplast phase.
The combined effects of heat and the aliphatic compounds tested indicated that the lipophilic groups in these compounds play a determinative role in the heat denaturation of thylakoids. The consequence of such structure alterations is inactivation of photosynthetic functions.
The lipophilic properties of the spinach thylakoid membrane as compared with certain artificial and natural membranes are described.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Y. Mukohata, T. Yagi, M. Higashida, K. Shinozaki and A. Matsuno,Plant Cell Physiol.,14 (1973), 111.
Y. Mukohata, M. Mitsudo, S. Kakumoto and M. Higashida,12 (1971), 869.
S. Katoh and A. San Pietro,Arch. Biochem. Biophys.,122 (1967), 144.
M. Kimimura and S. Katoh,Plant Cell Physiol.,13 (1972), 287.
T. Yamashita and W. L. Butler,Plant Physiol.,43 (1968), 2037.
Y. Mukohata, T. Yagi, A. Matsuno and M. Higashida,Plant Cell Physiol.,14 (1973), 119.
Y. Mukohata, in:Organization of Energy-transducing Membranes, L. Packer and M. Nakao (eds), (1973), Univ. of Tokyo Press, Tokyo, in press.
K. H. Buchel, W. Draber, A. Trebst and E. Pistorius,Z. Naturforsch,21b (1966) 243.
C. Hansch, E. J. Lien and F. Helmer,Arch. Biochem. Biophys.,128 (1968) 319.
D. I. Arnon,Plant Physiol.,24 (1949) 1.
C. Hansch, J. E. Quinlan and G. L. Lawrence,J. Org. Chem.,33 (1968) 347.
F. Helmer, K. Kiehs and C. Hansch,Biochemistry,7 (1968) 2858.
K. Asada, M. Takahashi and M. Urano,Anal. Biochem.,141 (1972) 311.
R. E. McCarty and C. H. Coleman,Arch. Biochem. Biophys.,141 (1970) 198.
J. J. Wolken and F. A. Schwertz,J. Gen. Physiol.,37, (1953) 111.
W. Junge and H. T. Witt,Z. Naturforsch,23b (1968) 244.
W. Kreutz and W. Menke,,17b (1962) 675.
J. C. Skou,Biochim. Biophys. Acta,30 (1958) 625.
P. Seeman,Biochem. Pharmacol.,15 (1966), 1632.
J. Gutknecht and D. C. Tosteson,J. Gen. Physiol.,55 (1970) 359.
S. J. Paterson, K. W. Butler, P. Huang, J. Labelle and I. C. P. Smith,Biochim. Biophys. Acta,266, (1972) 597.
C. Hansch,Accounts Chem. Res.,2 (1969) 232.
G. Rouser, G. J. Nelson, S. Fleischer and G. Simon, in:Biological Membranes, D. Chapman (ed.), (1968), pp. 5, Academic Press, London and New York.
L. A. Staehelin and M. C. Probine,Adv. Bot. Res.,3 (1970) 1.
S. J. Singer and G. L. Nicolson,Science,175 (1972) 720.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mukohata, Y., Yagi, T., Higashida, M. et al. Lipophilic interaction between thylakoid membranes and aliphatic compounds. J Bioenerg Biomembr 4, 479–490 (1973). https://doi.org/10.1007/BF01515940
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01515940