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Endogenous nitric oxide and prostaglandin E2 do not regulate the synthesis of each other in interleukin-1β-stimulated rat articular cartilage

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Abstract

Increased levels of nitric oxide (NO) and prostaglandins (PG) are present in the synovial fluid from patients with rheumatoid arthritis and osteoarthritis. Interleukin-1β (IL-1) has been shown to induce the synthesis of both of these mediators. The present work was designed to study the interactions of NO and PGE2 synthesis induced by IL-1 in rat articular cartilage. Incubation of intact cartilage with IL-1 resulted in different dose response curves for NO and PGE2 synthesis. Two inhibitors of nitric oxide synthase N-monomethyl-L-arginine (L-NMMA) and L-N-iminoethyl-ornithine, (L-NIO), abolished the IL-1-induced nitrite production but failed to have any influence on the PGE2 synthesis. Exogenous NO, produced by two chemically different NO-releasing compounds (SIN-1 and GEA 3175) had no effect on PGE2 synthesis in articular cartilage. Dexamethasone and ketoprofen inhibited IL-1 induced PGE2 production, while nitrite synthesis remained unaltered. Acetylsalisylic acid (ASA) reduced PGE2 synthesis and had a slight inhibitory action also on NO production. In conclusion, our results show, that IL-1 induces the synthesis of both PGE2 and NO in articular cartilage but these two inflammatory mediators are not mediating the synthesis of one another.

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Authors and Affiliations

  1. Medical School, University of Tampere, P. O. Box 607, SF-33I01, Tampere, Finland

    Tero A. H. Järvinen, Teemu Moilanen, Teppo L. N. Järvinen & Eeva Moilanen

  2. Institute of Medical Technology, University of Tampere, P. O. Box 607, SF-33I01, Tampere, Finland

    Tero A. H. Järvinen & Teppo L. N. Järvinen

  3. Departments of Surgery, University Hospital of Tampere, Tampere, Finland

    Teemu Moilanen

  4. Clinical Chemistry, University Hospital of Tampere, Tampere, Finland

    Eeva Moilanen

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  1. Tero A. H. Järvinen
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Järvinen, T.A.H., Moilanen, T., Järvinen, T.L.N. et al. Endogenous nitric oxide and prostaglandin E2 do not regulate the synthesis of each other in interleukin-1β-stimulated rat articular cartilage. Inflammation 20, 683–692 (1996). https://doi.org/10.1007/BF01488804

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