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Comparative effect of C3a and C5a on adhesion molecule expression on neutrophils and endothelial cells

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Abstract

Complement activation is known to enhance neutrophil binding to human umbilical vein endothelial cells (HUVECs). Recently, we have shown that recombinant human C5a upregulates P-selectin in HUVECs. Unstimulated human neutrophil binding is also increased on C5a stimulated HUVECs. We demonstrate in this report that C5a upregulates CD11b/CD18 in human neutrophils. Also shown is that synthetic C3a57–77 and an analog 15 amino acid C3a peptide (C3a15) neither upregulate CD11b/CD18 nor do the C3a peptides increase P-selectin, ICAM-1 or E-selectin in HUVECs. Thus C5a and not C3a is responsible for early (∼30 minutes) neutrophil adhesion to endothelial cells after complement activation.

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References

  1. Hugli, T. E., andH. J. Müller-Eberhard. 1978. Anaphylatoxins: C3a and C5a.Adv. Immunol. 261:1–53.

    Google Scholar 

  2. Bitter-Suermann, D. 1987. The anaphylatoxins.In The Complement System. Springer-Ver-lag, Berlin, 367–393.

    Google Scholar 

  3. Yancey, K. B., J. O'shea, T. Chused, E. Brown, T. Takahashi, M. M. Frank, andT. J. Lawley. 1985. Human C5a modulates monocyte Fc and C3 receptor expression.J. Immunol. 135:465–470.

    Google Scholar 

  4. Arnaout, M. A., R. M. Hakin, R. F. Todd, N. Dana, andH. R. Colten. 1985. Increased expression of an adhesion promoting surface glycoprotein in the granulocytopenia of hemodialysis.N. Eng. J. Med. 312:457–462.

    Google Scholar 

  5. O'shea, J. J., E. J. Brown, B. E. Seligmann, J. A. Mètcalf, M. Frank, andJ. I. Gallin. 1985. Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils.J. Immunol. 134:2580–2587.

    Google Scholar 

  6. Foreman, K. E., A. A. Vaporciyan, B. K. Bonish, M. L. Jones, K. J. Johnson, M. M. Glovsky, S. M. Eddy, andP. A. Ward. 1994. C5a induced expression of P-selectin in endothelial cells.J. Clin. Invest. 94:1147–1155.

    Google Scholar 

  7. Hattori, R., K. K. Hamilton, R. P. McEver, andP. J. Sims. 1989. Complement proteins C5b-C9 induce secretion of high molecular weight multimers or endothelial von Willebrand factor and translocation of granule membrane protein GMP-140 to the cell surface.J. Biol. Chem. 264:9053–9060.

    Google Scholar 

  8. Morganroth, M. L., S. O. Schoeneich, G. O. Till, P. A. Ward, S. J. Hovarth, andM. M. Glovsky. 1990. C3a57-77, a C-terminal peptide, causes thromboxane dependent pulmonary vascular constriction in isolated perfused rat lungs.Am. Rev. Respir. Dis. 141:296–300.

    Google Scholar 

  9. Ember, J. A., N. L. Johansen, andT. E. Hugli. 1991. Designing synthetic superagonists of C3a anaphylotoxin.Biochemistry (U.S.)30:3603–3612.

    Google Scholar 

  10. Mulligan, M. S., J. Varani, M. K. Dame, C. L. Lane, C. W. Smith, D. C. Anderson, andP. A. Ward. 1991. Role of endothelial-leukocyte adhesion molecule 1 (ELAM-1) in neutrophil-mediated lung injury in rats.J. Clin. Invest. 88:1396–1406.

    Google Scholar 

  11. Jaffe, E. A. Culture and identification of large vessel endothelial cells.In Biology of Endothelial Cells. E. A. Jaffe, editor. Martinus Nijoff, The Hague. 1–13.

  12. Vaporciyan, A. A., M. L. Jones, andP. A. Ward. 1993. Rapid analysis of leukocyteendothelial adhesion.J. Immunol. Methods 159:93–100.

    Google Scholar 

  13. Mulligan, M. S., A. A.Vaporciyan, M.Miyasaka, T.Tamatani, and P. A.Ward. Tumor necrosis factorα regulatedin vivo intrapulmonary expression of ICAM-1.Am. J. Path. 142:1739–1749.

  14. Marks, R. M., R. F. Todd, III, andP. A. Ward. 1989. Rapid induction of neutrophilendothelial adhesion by endothelial complement fixation.Nature 339:314–317.

    Google Scholar 

  15. Glovsky, M. M., T. E. Hugli, T. Ishizaka, L. M. Lichtenstein, B. W. Erickson. 1979. Anaphylatoxin-induced histamine release with human leukocytes: Studies of C3a leukocyte binding and histamine release.J. Clin. Invest. 64:804–811.

    Google Scholar 

  16. Mulligan, M. S., C. W. Smith, D. C. Anderson, R. F. Todd, M. Miyasaka, T. Tamatani, T. B. Issekutz, andP. A. Ward. 1993. Role of leukocyte adhesion molecules in complement-induced lung injury.150:2401–2406.

    Google Scholar 

  17. Mulligan, M. S., J. Varani, J. S. Warren, G. O. Till, C. W. Smith, D. C. Anderson, R. F. Todd, andP. A. Ward. 1992. Role ofβ2 integrins of rat neutrophils in complement-and oxygen radical-medicated acute inflammatory injury.J. Immunol. 148:1847–1857.

    Google Scholar 

  18. Mulligan, M. S., M. J. Polley, R. J. Bayer, M. F. Nunn, J. C. Paulson, andP. A. Ward. 1992. Neutrophil-dependent acute lung injury. Requirement for P-selectin (GMP-140).J. Clin. Invest. 90:160–167.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, University of Michigan, Ann Arbor, Michigan

    K. E. Foreman, R. L. Warner & P. A. Ward

  2. Parke-Davis Research Division of Warner-Lambert Company, Ann Arbor, Michigan

    K. E. Foreman

  3. Asthma and Allergy Center, Huntington Memorial Hospital, Pasadena, California

    M. M. Glovsky

  4. Caltech Beckman Institute, Pasadena, California

    S. J. Horvath

Authors
  1. K. E. Foreman
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  2. M. M. Glovsky
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  3. R. L. Warner
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  4. S. J. Horvath
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  5. P. A. Ward
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Foreman, K.E., Glovsky, M.M., Warner, R.L. et al. Comparative effect of C3a and C5a on adhesion molecule expression on neutrophils and endothelial cells. Inflammation 20, 1–9 (1996). https://doi.org/10.1007/BF01487740

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