Zusammenfassung
Membranen unbehandelter Erythrocyten sowie nach Einwirkung von hypotonen Lösungen und von Saponin wurden mit der Gefrierätztechnik präpariert und elektronenmikroskopisch untersucht. Wird Glycerin als Frostschutzmittel verwendet, so stellen sich eine innere und eine äußere Membranoberfläche dar, die unterschiedlich dicht mit etwa 50–120 Å großen Partikeln bedeckt sind. Diese Partikel sind durch Behandlung mit hypotonen Lösungen, Saponin und Zentrifugation nicht zu entfernen. Wird auf Glycerin verzichtet, so sind die beschriebenen Oberflächen zusätzlich stellenweise von einem Film eines homogenen Materials bedeckt. Werden die Erythrocyten in hypotone Lösungen gebracht, so erfolgt der Hämoglobin-Austritt innerhalb weniger Sekunden durch eine 0,5–1,5 µ große Öffnung in der Zellmembran. Weiteres Verbleiben in hypotonen Lösungen und Saponineinwirkung führen zur Ausbildung blasiger Membranausstülpungen. Selten wird durch Saponin eine mosaikartige Membranstruktur sichtbar gemacht. Eine Deutung der Befunde im Hinblick auf den Aufbau der Erythrocytenmembran wird versucht.
Summary
The membranes of untreated erythrocytes as well as after treatment with hypotonic solutions and saponin were prepared by freezeetching, and examined with the electron microscope. When glycerine was used as cryoprotective substance, an internal and an external membrane surface were exhibited, covered with particles of about 50–120 A. These particles could not be removed by treatment with hypotonic solutions, by saponin or centrifugation. When no glycerine was used, the membrane surfaces described before, in addition were covered sporadically with an film of an homogeneous material. After treatment with hypotonic solutions the expulsion of hemoglobin was effected through an opening of 0,5–1,5 µ in the cell membrane within a few seconds. Further treatment with hypotonic solutions and saponin caused membrane evaginations. In some cases saponin produced a mosaic-like membrane structure. An interpretation of the findings with regard to the structure of the erythrocyte membrane is attempted.
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Studie im Rahmen der Assoziation Hämatologie-EURATOM-GSF Nr. 031-64/BIAD.
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Huhn, D., Pauli, G.D. & Grassmann, D. Die Erythrocytenmembran. Klin Wochenschr 48, 939–943 (1970). https://doi.org/10.1007/BF01487635
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DOI: https://doi.org/10.1007/BF01487635