Zusammenfassung
Diaminooxidase (DAO, Histaminase) ist nach Schayer ein wesentliches Enzym im Histaminabbau. Es metabolisiert ferner eine Reihe von Diaminen wie Putrescin und Cadaverin und wird mit der Histaminase für identisch gehalten. Lipoproteinlipase (LL) hingegen ist ein Schlüsselenzym im Fettmetabolismus. Beide Enzyme können bei Vertebraten durch parenteral appliziertes Heparin dosisabhängig aus den Enzymdepots in das Blutplasma freigesetzt werden Die Plasmaspiegel der DAO und LL wurden nach Heparingabe (200 U/kg KG, i.v.) bei 30 Patienten bestimmt. Es handelte sich dabei um 20 verschiedene, überwiegend entzündliche Erkrankungen. In allen Fällen fand sich eine deutlich verringerte Freisetzung der Postheparin-Diaminooxidase (PHD) und der Postheparin-Lipoproteinlipase (PHLA). Zwischen der Aktivität der freigesetzten beiden Enzyme bestand eine hochsignifikante Korrelation (r=0,843,p<0,0005). Bei allen Patienten lagen normale Insulinspiegel vor, ferner bestand keine Korrelation zwischen der PHLA und den Plasma-Triglyceridspiegeln. Die Verringerung der PHD bzw. PHLA war ein empfindlicherer biochemischer Parameter als der Anstieg der Serum-Glutamat-Pyruvat-Transaminase (SGPT) bei akuter Hepatitis bzw. des C-reaktiven Proteins (CRP) bei chronisch entzündlichen Nierenerkrankungen. Dieser Befund ist wahrscheinlich ein allgemeines Phänomen bei entzündlichen Erkrankungen. Neben der bekannten hormonellen Regulation der PHLA müssen entzündungsbedingte Einflüsse auf dieses Enzym diskutiert werden.
Summary
Diamine oxidase (DAO, histaminase), according to Schayer, is an essential enzyme in histamine metabolism. It metabolises also a variety of diamines such as putrescine and cadaverine and is generally accepted to be identical with histaminase. Lipoproteinlipase (LL) is assumed to play an essential role in lipid metabolism. In vertebrates, parenterally applied heparin causes a marked dose-dependent rise of the plasma level of both enzymes mainly due to the release from the enzyme containing organs. The plasmatic level changes of DAO and LL after heparin application (200 U/kg b.wt., i.v.) have been studied in 30 patients suffering from 20 different, predominantly inflammatory diseases. In all cases the release of postheparin diamine oxidase (PHD) and of post-heparin lipoproteinlipase (PHLA) was found to be markedly decreased. There existed a highly significant correlation between the degree of the release of both enzymes (r=0.843,p<0.0005). In all patients normal levels of plasma insulin were detected. No correlation was found between PHLA and plasma triglycerides levels. Decrease of PHD respectively PHLA was a more sensitive biochemical parameter than were changes of serum glutamic pyruvic transaminase in acute hepatitis and of C reactive protein (CRP) in chronic inflammatory kidney diseases. This finding is most likely a general phenomenon in inflammatory diseases. Besides hormonal regulation inflammation-dependent effects on PHLA have to be discussed.
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Gäng, V., Gehring, G. & Schönborn, J. Combined decrease of postheparin-diamine oxidase (histaminase) and postheparin-lipoproteinlipase in inflammatory diseases. Klin Wochenschr 55, 169–173 (1977). https://doi.org/10.1007/BF01469137
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DOI: https://doi.org/10.1007/BF01469137