Abstract
Fetal cells obtained from amniotic fluid at various stages of pregnancy were successfully cultivated. Quantitative enzyme analysis and qualitative enzyme analysis, utilizing starch gel electrophoresis, were performed. Increased glucose 6-phosphate dehydrogenase activity associated with a decreased percentage of sex chromatin positive cells were found in cells derived from two 10-week female fetuses. After 6 weeks, these cultures contained normal levels of glucose 6-phosphate dehydrogenase activity and normal numbers of sex chromatin positive cells. Qualitative changes of glucose 6-phosphate dehydrogenase and lactate dehydrogenase were demonstrated.
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This study is supported by U.S.P.H.S. Grants 1 RO1 HD 02752 and TI AM 5186.
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Nadler, H.L. Patterns of enzyme development utilizing cultivated human fetal cells derived from amniotic fluid. Biochem Genet 2, 119–126 (1968). https://doi.org/10.1007/BF01458711
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DOI: https://doi.org/10.1007/BF01458711