Abstract
Epithelial basement membrane in oral carcinomas was examined by immunoelectron microscopy using anticollagen type IV and antilaminin antibodies. Intense and irregularly increased immunostaining was observed at the boundary between carcinoma cells and stromal tissue. Heterotopic immunostaining was found in the enlarged intercellular spaces of peripheral carcinoma cells. In addition, rough endoplasmic reticulum (rER) and vesicles in these cells were frequently stained. These findings suggest an increased synthesis and secretion of basement membrane components at the invading edge of the tumor, and also suggest that some oral carcinomas may spread into the surrounding tissue without apparent loss of the basement membrane.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Martinez-Hernandez A, Amenta PS (1983) The basement membrane in pathology. Lab Invest 48:656–677
Daher N, Abourachid H, Bove N, Petit J, Burtin P (1987) Collagen IV staining pattern in bladder carcinomas: Relationship to prognosis. Br J Cancer 55:665–671
Albrechtsen R, Nielsen M, Wewer U, Engvall E, Ruoslahti E (1981) Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin. Cancer Res 41:5076–5081
Liotta LA, Rao C, Barsky SH (1983) Tumor invasion and the extracellular matrix. Lab Invest 49:636–649
Bosman FT, de Bruine A, Flohil C, van der Wurff A, Ten Kate J, Diniens WW (1993) Epithelial-stromal interactions in colon cancer. Int J Dev Biol 37:203–211
Barsky SH, Siegal G, Jannotta F, Liotta LA (1983) Loss of basement membrane components by invasive tumours but not by their benign counterparts. Lab Invest 49:140–147
Sinha AA, Gleason DF, Wilson MJ, Staley NA, Furcht LT, Palm SL, Reddy PK, Sibley RK, Martinez-Hernandez A (1989) Immunohistochemical localization of laminin in the basement membranes of normal, hyperplastic, and neoplastic human prostate. Prostate 15:299–313
Ljubimov AV, Bartek J, Couchman JR, Kapuller LL, Veselov VV, Kovarik J, Perevoshchikov AG, Krutovskikh VA (1992) Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50:562–566
Orita H, Korenaga D, Maehara Y, Baba H, Sugimachi K (1993) Laminin distribution patterns are closely related to liver metastasis in gastric cancer. Cancer 71:1201–1206
Hunt G (1989) The role of laminin in cancer invasion and metastasis. Exp Cell Biol 57:165–176
Castronovo V, Bracke ME, Mareel MM, Reznik M, Foidart JM (1991) Absence of laminin deposition in breast cancer and metastases except to the brain. Pathol Res Pract 187:201–208
Fisseler EA, Prebeg M, Voss B, Muller KM (1990) Extracellular matrix in preneoplastic lesions and early cancer of the lung. Pathol Res Pract 186:95–101
Mori M, Shimono R, Kido A, Kuwano H, Akazawa K, Sugimachi K (1991) Distribution of basement membrane antigens in human esophageal lesions: an immunohistochemical study. Int J Cancer 47:839–842
Antonelli AR, Nicolai P, Cappiello J, Peretti G, Molinari TM, Rosa D, Grigolato PG, Favret M, Maroccolo D (1991) Basement membrane components in normal, dysplastic, neoplastic laryngeal tissue and metastatic lymph nodes. Acta Otolaryngol Stockh 111:437–443
Kumagai S, Kojima S, Imai K, Nakagawa K, Yamamoto E, Kawahara E, Nakanishi I (1994) Immunohistologic distribution of basement membrane in oral squamous cell carcinoma. Head Neck 16:51–57
Bouziges F, Simo P, Simon AP, Haffen K, Kedinger M (1991) Altered deposition of basement-membrane molecules in cocultures of colonic cancer cells and fibroblasts. Int J Cancer 48:101–108
Carter RL (1985) Immunohistochemical localization of basement membrane type IV collagen in invasive and metastatic squamous cell carcinoma of the head and neck. J Pathol 147:159–164
Laurie GW, Leblond CP, Martin GR (1982) Intercellular localization of basement membrane precursors in the endodermal cells of the rat parietal yolk sac: II. Immunostaining for type IV collagen and its precursors. J Histochem Cytochem 30:983–990
Laurie GW, Leblond CP, Martin GR, Silver MM (1982) Intercellular localization of basement membrane precursors in the endodermal cells of the rat parietal yolk sac. III Immunostaining for laminin and its precursors. J Histochem Cytochem 30:991–998
Kadoya Y, Yamashina S (1991) Reconstruction of the basement membrane in a cultured submandibular gland. Anat Embryol (Berl) 183:491–499
Horiguchi Y, Maruguchi T, Maruguchi Y, Suzuki S, Fine JD, Leigh IM, Yoshiki T, Ueda M, Toda KI, Isshiki N (1994) Ultrastructural and immunohistochemical characterization of basal cells in three-dimensional culture models of the skin. Arch Dermatol Res 286:53–61
Jiang D-J, Wilson DF, Smith PS, Pierce AM, Wiebkin OW (1994) Distribution of basal lamina type IV collagen and laminin in normal rat tongue mucosa and experimental oral carcinoma: Ultrastructural and immunolocalisation and immunogold quantitation. Oral Oncol Eur J Cancer 30B:237–243
Ingber DE, Madri JA, Jamieson JD (1981) Role of basal lamina in neoplastic disorganization of tissue architecture. Proc Natl Acad Sci USA 78:3901–3905
Turpeenniemi-Hujanen T, Thorgeirrsson UP, Hart IR (1985) Expression of collagenase IV (basement membrane collgenase) activity in murine tumour cell hybrids that differ in metastatic potential. J Natl Cancer Inst 74:99–103
Sloane BF (1990) Cathepsin B and cystatins: evidence for a role in cancer progression. Semin Cancer Biol 1:137–152
Liotta LA, Tryggvason K, Garbisa S, Hart I, Foltz CM, Shafie S (1980) Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284:67–68
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ueno, S., Osugi, Y., Nishimura, A. et al. Ultrastructural localization of collagen type IV and laminin expression in the epithelial basement membrane of oral carcinomas. Med Electron Microsc 30, 37–42 (1997). https://doi.org/10.1007/BF01458350
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01458350