Riassunto
È stato studiato l'effetto dell'insulina sul consumo di glucosio e produzione di CO2 da parte del tessuto adiposo umano in diverse condizioni sperimentali.
L'anestesia ha dimostrato di modificarne il comportamento in modo poco sensibile.
Non sono state riscontrate significative differenze nel consumo di glucosio da parte del tessuto adiposo proveniente da diversi distretti.
La concentrazione di glucosio nelmedium di preincubazione influenza significativamente il consumo di glucosio durante l'incubazione. È stato dimostrato, con l'impiego della tecnica manometrica e con l'uso di glucosio-1-C14, un aumento della produzione di CO2 da parte del tessuto adiposo umano per effetto dell'insulina.
Tale aumento raggiunge tuttavia valori molto modesti, sensibilmente inferiori a quelli osservati con tessuto adiposo di ratto.
Resume
L'on a étudié l'effet de l'insuline sur la consommation de glucose et la production de CO2 obtenues du tissu adipeux humain en différentes conditions d'expériences.
L'anésthésie a démontré qu'elle en modifiait le comportement d'une façon peu sensible.
On n'a pas constaté des différences sensibles et significatives dans la consommation de glucose opérée par le tissu adipeux provenant de différentes zone organiques.
La concentration du glucose dans lemédium de préincubation influence significativement la consommation du glucose pendant l'incubation. Il a été prouvé, par l'emploi de la technique manométrique et l'utilisation du glucose-1-C14, une augmentation de la production de CO2 par le tissu adipeux humain à la suite de l'effet de l'insuline.
Cette augmentation atteint toutefois des valeurs très modestes, sensiblement inférieures de celles observées avec un tissu adipeux de rat.
Resumen
Ha sido estudiado el efecto de la insulina sobre el consumo de la glucosa y la producciòn de CO2 de parte del tejido adiposo humano en diferentes condiciones experimentales.
La anestesia ha demostrado de modificar escarsamente su comportamiento.
No han sido averiguadas marcadas diferencias en el consumo de la glucosa de parte del tejido adiposo proveniente de distintos territorios.
La concentraciòn de la glucosa en elmedium de preincubaciòn influencia significativamente el consumo de la glucosa durante la incubaciòn.
Ha sido probado que, con el empleo de la tecnica manométrica y con el uso de la glucosa-1-C14, aumenta la producciòn de CO2 de parte del tejido adiposo humano por el efecto de la insulina.
Ese incremento alcanza sinembargo valores muy pequeños, sensiblemente inferiores a aquellos observados con tejido adiposo del ratón.
Zusammenfassung
Es wurde der Effekt des Insulins auf den Glukoseverbrauch und die CO2-Produktion seitens des humanen Fettgewebes unter verschiedenen experimentellen Bedingungen untersucht.
Es zeitigte sich, dass die Anaesthesie das Verhalten wenig veraendert.
Es wurden keine signifikanten Unterschiede im Glukoseverbrauch seitens Fettgewebes aus verschiedenen Distrikten festgestellt. Die Glukosekonzentration im Praeinkubations-Medium beeinflusst den Glukoseverbrauch waehrend der Inkubation signifikant. Mittels der manometrischen Technik und bei Verwendung von 1-C14Glukose wurde eine Zunahme der CO2-Produktion seitens des humanen Fettgewebes unter der Wirkung des Insulins ermittelt.
Diese Zunahme erreicht jedoch sehr spaerliche Werte, die bedeutend geringer sind als die, welche man mit Rattenfettgewebe beobachtet.
Summary
The effect of insulin on glucose uptake and CO2 production by human adipose tissue has been studied in various experimental conditions.
Is has been shown that anesthesia modifies the behaviour to only a slight extent.
The glucose uptake on the part of the adipose tissue of various body regions has not shown significant differences.
The concentration of glucose in the pre-incubationmedium significantly influences glucose consumption during incubation.
Increase of the CO2 production on the part of human adipose tissue, under the influence of insulin, has been demonstrated using the manometric technique and glucose-1-C14.
This increase, however, reaches only slight values, sensibly inferior to those observed with adipose tissue in the rat.
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Pozza, G., Ghidoni, A. & Tarenzi, L. Metabolismo del tessuto adiposo umano incubato in vitro I - effetto dell'insulina sul consumo di glucosio e produzione di CO2 in diverse condizioni sperimentali. Acta diabet. lat 1, 170–177 (1964). https://doi.org/10.1007/BF01457534
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DOI: https://doi.org/10.1007/BF01457534