Summary
Equilibrium binding assays of EGF were performed on confluent cultures of 12 human meningiomas at early passage. In all meningeomas complete binding curves were obtained and the resulting ED 50 values ranged between 0.5 and 6.3 nM. In four cases (ED 50 values ranging from 1.5 nM to 3.0 nM) where saturation analysis was performed, the sites were saturable at similar levels (7nM). In five cases additional experiments were undertaken to evaluate the biological response of cultured cells to EGF as assessed by3H-thymidine incorporation. In all cases EGF was a potent stimulus and increased3H-thymidine incorporation by 2.5 to 6-fold. Functionally intact EGF receptors appear to be a regular feature of meningiomas in cell culture and appear not to be related to histological classification.
Similar content being viewed by others
Abbreviations
- EGF:
-
epidermal growth factor
- FGF:
-
fibroblast growth factor
- FCS:
-
foetal calf serum
- TGF:
-
transforming growth factor
References
Carpenter G, Cohen S (1979) Epidermal growth factor. Ann Rev Biochem 48: 193–216
Chua CC, Geiman DE, Schreiber AB, Ladda RL (1984) Nonfunctional epidermal growth factor receptor in cells transformed by Kirsten sarcoma virus. Biochem Biophys Res Commun 118: 538–547
Cohen S, Savage CR (1975) Preparation of epidermal growth factor. Methods in Enzymol 37: 170–176
Costero I, Pomerat CM, Jackson IJ, Borroso-Moguel R, Cevez AZ (1955) Tumors of the human nervous system in tissue culture. I. The cultivation and cytology of meningoema cells. J Natl Cancer Inst 15: 1319–1339
DeLean A, Munson PJ, Rodbard D (1978) Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay and physiological dose response curves. Am J Physiol 235 (2): E97-E102
Downward J, Yarde Y, Mayes E, Scrace G, Totty N, Stockwell P, Ullrich A, Schlessinger J, Waterfield MD (1984) Close similarity of epidermal growth factor receptor and verb-B oncogene protein sequences. Nature 307: 521–527
Gospodarowicz D, Moran JS (1976) Growth factors in mammalian cell culture. Ann Rev Biochem 45: 531–558
Gospodarowicz D, Cheng J, Lui GM, Baird A, Böhlen P (1984) Isolation of brain fibroblast growth factor by heparin Sepharose chromatography: identity with pituitary fibroblast growth factor. Proc Natl Acad Sci USA 81: 6963–6967
Hunter WM, Greenwood PC (1962) Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194: 195–196
Liberman TA, Nusbaum HR, Razon N, Kris R, Lax I, Soreq H, Whittle N, Waterfield MD, Ullrich A, Schlessinger J (1985) Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumors of glial origin. Nature 313: 144–147
Marquardt H, Hunkapiller MW, Hood LE, Twardzik DR, DeLarco JE, Stephenson JR., Todaro GJ (1983) Transforming growth factors produced by retrovirus-transformed rodent fibroblasts and human melanoma cells: amino acid sequence homology with epidermal growth factor. Proc Natl Acad Sci USA 80: 4684–4688
Massague J (1983) Epidermal growth factor-like transforming growth factor. J Biol Chem 258: 13614–13620
Rodbard D, Frazier GR (1975) Statistical analysis of radioligand assay data. Methods in Enzymol 37: 3–22
Rozengurt E (1983) Growth factors, cell proliferation and cancer: an overview. Mol Biol Med 1: 169–181
Weiner RI, Bethea CL, Jaquet PJ, Ramsdell JS, Gospodarowicz DJ (1983) Culture of dispersed pituitary cells on extracellular matrix. Methods in Enzymol 103: 287–293
Westermark B, Magnusson A, Heldin C-H (1982) Effect of epidermal growth factor on membrane motility and cell locomotion in cultures of clonal human glioma cells. J Neurosci Res 8: 491–507
Westphal M, Harsh GR, Rosenblum ML, Hammonds RG (1985) Epidermal growth factor receptors in human glioblastoma cells differ from those in epidermal carcinoma cells. Biochem Biophys Res Commun, in press
Xu Y-H, Richert N, Ito S, Merlino GT, Pastan I (1984) Characterisation of epidermal growth factor receptor gene expression in malignant and normal human cell lines. Proc Natl Acad Sci USA 81: 7308–7312
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Dr. Friedrich Loew on the occasion of his 65th birthday and the 25th anniversary of the Homburg Neurosurgical University Clinic, which has been founded and built up by him.
Rights and permissions
About this article
Cite this article
Westphal, M., Herrmann, H.D. Epidermal growth factor—Receptors on cultured human meningioma cells. Acta neurochir 83, 62–66 (1986). https://doi.org/10.1007/BF01420510
Issue Date:
DOI: https://doi.org/10.1007/BF01420510